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一次性尿布中的尿液样本:一种用于尿培养的准确方法。

Urine samples from disposable diapers: an accurate method for urine cultures.

作者信息

Cohen H A, Woloch B, Linder N, Vardi A, Barzilai A

机构信息

Pediatric Ambulatory Center, Sackler School of Medicine, Tel Aviv University, Petach Tikvah, Israel.

出版信息

J Fam Pract. 1997 Mar;44(3):290-2.

PMID:9071249
Abstract

BACKGROUND

The method of collection of the urine sample is of paramount importance in making a diagnosis of urinary tract infection in infants and children. Squeezing urine out of disposable diapers can provide a urine sample that can be used to detect chemical abnormalities as well as a specimen suitable for microscopic examination. To date there have been no reported studies on the use of this technique for urine culture as compared with samples collected by suprapubic aspiration and catheterization.

METHODS

Urine was obtained from 38 infants aged under 2 years who presented with fever with no obvious cause. All infants had urine collected either by catheterization or suprapubic aspiration and by extraction from a disposable diaper. The urine samples were cultured using standard bacteriologic techniques.

RESULTS

Five infants had a urinary tract infection, as shown by a pure growth of more than 10(5) colonies/mL of a single species of bacterium. In all the cases the same result was obtained from both the diaper urine sample and the sample obtained by suprapubic aspiration or catheter. In 31 infants the urine samples collected by both techniques (diaper and catheter or suprapubic aspiration) were negative, and in only 2 infants did the diaper specimen yield a positive result, while the urine obtained by suprapubic aspiration or catheter was sterile.

CONCLUSIONS

Urine obtained from a disposable diaper can provide a valid sample for diagnosing urinary tract infection. The technique is simple, and can be carried out readily in ambulatory settings with minimal equipment and expense.

摘要

背景

尿液样本的采集方法对于婴幼儿尿路感染的诊断至关重要。从一次性尿布中挤出尿液可提供用于检测化学异常的尿液样本以及适合显微镜检查的标本。与通过耻骨上穿刺抽吸和导尿收集的样本相比,迄今为止尚无关于使用该技术进行尿培养的报道研究。

方法

从38名2岁以下无明显原因发热的婴儿中获取尿液。所有婴儿的尿液均通过导尿或耻骨上穿刺抽吸以及从一次性尿布中提取收集。尿液样本采用标准细菌学技术进行培养。

结果

5名婴儿患有尿路感染,表现为单一菌种的纯培养菌落数超过10⁵/mL。在所有病例中,尿布尿液样本与耻骨上穿刺抽吸或导尿获得的样本结果相同。31名婴儿通过两种技术(尿布和导尿或耻骨上穿刺抽吸)收集的尿液样本均为阴性,只有2名婴儿的尿布标本呈阳性结果,而耻骨上穿刺抽吸或导尿获得的尿液无菌。

结论

从一次性尿布中获取的尿液可为诊断尿路感染提供有效的样本。该技术简单,在门诊环境中使用最少的设备和费用即可轻松进行。

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