Savage C O, Brooks C J, Adu D, Richards G, Howie A J
Department of Medicine (CCRIS), Medical School, University of Birmingham, U.K.
J Pathol. 1997 Jan;181(1):111-5. doi: 10.1002/(SICI)1096-9896(199701)181:1<111::AID-PATH698>3.0.CO;2-9.
A glomerular and kidney organ culture method was developed to study cytokine inducibility of adhesion molecules and MHC antigen expression. Glomerular cells showed constitutive expression of intercellular cell adhesion molecule-1 (ICAM-1) and MHC class I and II antigens, but not vascular cell adhesion molecule-1 (VCAM-1), E-selectin, or P-selectin. Expression of E-selectin on glomerular endothelial cells (ECs) was induced by interleukin-1 (IL-1), tumour necrosis factor (TNF), interferon-gamma (IFN-gamma) and granulocyte/ macrophage-colony stimulating factor (GM-CSF); this induction was inhibited by transforming growth factor beta (TGF beta) and by IL-4. P-selectin expression was never seen within glomeruli. VCAM-1 was constitutively expressed by Bowman's capsule and proximal tubules and was weakly induced on glomerular ECs by TNF and IL-4 in combination. Glomerular endothelial ICAM-1 expression was increased by IL-1, TNF, IFN-gamma, and GM-CSF, while TGF beta inhibited induction by TNF and IL-1. Expression of MHC class I and II antigens by glomerular ECs was constitutive; further upregulation of MHC class II by IFN-gamma was observed. These studies suggest that leukocyte-endothelial cell interactions that occur within the kidney follow broadly similar principles as are proposed to occur elsewhere in the body but, in addition, there are subtle differences that reflect local conditions.
我们开发了一种肾小球和肾脏器官培养方法，以研究细胞因子对黏附分子诱导性及MHC抗原表达的影响。肾小球细胞组成性表达细胞间黏附分子-1（ICAM-1）以及MHC I类和II类抗原，但不表达血管细胞黏附分子-1（VCAM-1）、E-选择素或P-选择素。白细胞介素-1（IL-1）、肿瘤坏死因子（TNF）、干扰素-γ（IFN-γ）和粒细胞/巨噬细胞集落刺激因子（GM-CSF）可诱导肾小球内皮细胞（EC）表达E-选择素；转化生长因子β（TGFβ）和IL-4可抑制这种诱导作用。肾小球内未见P-选择素表达。Bowman囊和近端小管组成性表达VCAM-1，TNF和IL-4联合作用可使肾小球EC微弱诱导表达VCAM-1。IL-1、TNF、IFN-γ和GM-CSF可增加肾小球内皮ICAM-1表达，而TGFβ可抑制TNF和IL-1的诱导作用。肾小球EC组成性表达MHC I类和II类抗原；IFN-γ可进一步上调MHC II类抗原表达。这些研究表明，肾脏内发生的白细胞-内皮细胞相互作用与身体其他部位所提出的相互作用遵循大致相似的原则，但此外，还存在反映局部情况的细微差异。
