Devaney J M, Marino M A, Williams P E, Weaver K R, Del Rio S A, Turner K A, Belgrader P
Armed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, Rockville, MD 20850, USA.
Appl Theor Electrophor. 1996;6(1):11-4.
The analysis of crude polymerase chain reaction (PCR) products by capillary electrophoresis (CE) is often compromised by the presence of a high concentration of salt. Salt interferes with the electrokinetic injection and induces localized heating within the column, hence, PCR products must be desalted or cleaned-up prior to CE analysis. A variety of commercial clean-up systems are available that have been traditionally used to prepare PCR products for cloning, sequencing, and digestion with restriction enzymes. These systems were tested for their effectiveness in preparing PCR products for CE analysis and were evaluated based on CE resolution, salt removal, DNA recovery, processing time, and cost.
通过毛细管电泳（CE）分析粗制聚合酶链反应（PCR）产物时，常常会因高浓度盐的存在而受到影响。盐会干扰电动进样，并在柱内引起局部加热，因此，在进行CE分析之前，必须对PCR产物进行脱盐或纯化处理。有多种市售的纯化系统可供使用，传统上这些系统用于制备用于克隆、测序和用限制性内切酶消化的PCR产物。对这些系统在制备用于CE分析的PCR产物方面的有效性进行了测试，并根据CE分辨率、盐去除率、DNA回收率、处理时间和成本进行了评估。
