Siles B A, Collier G B, Reeder D J, May W E
Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Appl Theor Electrophor. 1996;6(1):15-22.
TreviGel-500, a new polysaccharide matrix containing AgaCryl, commercially available as a powder for slab gel electrophoresis, is now being applied to the separation of DNA fragments in capillary electrophoresis. The capillary mode allows the use of one to two orders of magnitude lower mass fractions of matrix and approximately five to six orders of magnitude lower sample quantities than the slab gel electrophoresis counterpart for optimal separation of DNA fragments in the 100 to 2,000 base pair size range. In the capillary mode, this new separation matrix forms a semi-rigid gel that demonstrates enhanced selectivity for DNA fragments in the 1,000 to 7,000 base pair size range relative to alternative size-sieving polymer solutions. In addition, this matrix offers the advantages of lower toxicity than acrylamide. Comparisons are drawn between the use of this matrix in both slab gel electrophoresis and capillary electrophoresis for the separation of DNA fragments with respect to the mass fraction of the matrix in buffer, the buffer composition and sample loading or injection parameters.
TreviGel - 500是一种含有AgaCryl的新型多糖基质，以用于平板凝胶电泳的粉末形式在市场上销售，目前正应用于毛细管电泳中DNA片段的分离。与平板凝胶电泳相比，毛细管模式允许使用低一到两个数量级的基质质量分数，以及低大约五到六个数量级的样品量，以便在100到2000碱基对大小范围内对DNA片段进行最佳分离。在毛细管模式下，这种新型分离基质形成一种半刚性凝胶，相对于其他尺寸筛分聚合物溶液，它对1000到7000碱基对大小范围内的DNA片段表现出更高的选择性。此外，这种基质具有比丙烯酰胺更低的毒性优势。本文对该基质在平板凝胶电泳和毛细管电泳中用于分离DNA片段时，在缓冲液中基质的质量分数、缓冲液组成以及样品上样或进样参数方面进行了比较。
