Svec P, Sedlácek I, Pakrová E
Ceská sbírka mikroorganismů, Prírodovĕdecká fakulta Masarykovy univerzity, Brno.
Epidemiol Mikrobiol Imunol. 1996 Dec;45(4):153-7.
Enterococci are part of the common microflora of man and are isolated in large numbers also from the environment. Recently their presence in clinical material is increasing and they have become an important causal agent of nosocomial infections. From human clinical material (urine, vaginal smears, wounds) a total of 164 strains of enterococci was isolated. Identification of these isolates by means of the commercial STREPTOtest kit was not very successful (71.3%). The use of several supplementary tests and evaluation by the TNW programme improved successful identification (98.8%). The dominating species in clinical material was E. faecalis (94.5%), the second most frequent isolated species was E. faecium (3%). To achieve better identification of enterococci the authors recommend to supplement the kit by further tests (acidification of arabinose, ribose and pyruvate assimilation).
肠球菌是人类常见微生物群落的一部分，也大量从环境中分离得到。最近它们在临床材料中的出现频率不断增加，已成为医院感染的重要病原体。从人类临床材料（尿液、阴道涂片、伤口）中总共分离出164株肠球菌。使用商业STREPTOtest试剂盒对这些分离株进行鉴定的成功率不高（71.3%）。通过几种补充试验并采用TNW程序进行评估提高了鉴定成功率（98.8%）。临床材料中占主导地位的菌种是粪肠球菌（94.5%），第二常见的分离菌种是屎肠球菌（3%）。为了更好地鉴定肠球菌，作者建议通过进一步试验（阿拉伯糖酸化、核糖和丙酮酸同化试验）对试剂盒进行补充。
