Svec P, Sedlácek I
Czech Collection of Microorganisms, Masaryk University, Brno, Czech Republic.
Folia Microbiol (Praha). 1999;44(1):3-10. doi: 10.1007/BF02816212.
We studied 630 bacterial strains isolated from surface waters and determined as enterococci on the basis of their growth on Slanetz-Bartley agar in typical colonies. The strains were tested and characterized by several key conventional tests for basic differentiation of enterococci and by commercial test kits. We identified 135 strains of E. foecium (21%), 115 E. faecalis (18%), 30 E. mundtii (5%), 27 E. hirae (4%), 22 E. casseliflavus (3%), 21 E. gallinarum (3%), 17 E. durans-E. hirae complex (3%), 5 E. durans (1%), and 1 strain of E. avium. 150 strains were classified only as Enterococcus sp. (25%) and 107 strains (17%) isolated from Slanetz-Bartley agar were not enterococci. We found that the non-enterococcal group consisted of other Gram-positive cocci and Gram-positive and Gram-negative rods. Based on the identification we tried to find a relation between taxonomic position of isolated strains and their colony morphology on Slanetz-Bartley agar. Our of the total of 523 identified enterococci, 345 strains (66%) formed purple colonies, 136 red colonies (26%), 37 pink colonies (7%) and 5 cream colored colonies (1%). There was no correlation among the color, size or colony morphology and the taxonomic characterization of enterococcal strains.
我们研究了从地表水分离出的630株细菌菌株，这些菌株基于其在Slanetz-Bartley琼脂上典型菌落的生长情况被鉴定为肠球菌。通过几种用于肠球菌基本鉴别分型的关键常规试验以及商业检测试剂盒对这些菌株进行了检测和特征分析。我们鉴定出135株屎肠球菌（21%）、115株粪肠球菌（18%）、30株蒙氏肠球菌（5%）、27株海氏肠球菌（4%）、22株格氏肠球菌（3%）、21株鹑鸡肠球菌（3%）、17株耐久肠球菌-海氏肠球菌复合体（3%）、5株耐久肠球菌（1%）以及1株鸟肠球菌。150株仅被分类为肠球菌属（25%），从Slanetz-Bartley琼脂分离出的107株（17%）并非肠球菌。我们发现非肠球菌组由其他革兰氏阳性球菌以及革兰氏阳性和革兰氏阴性杆菌组成。基于鉴定结果，我们试图找出分离菌株的分类学位置与其在Slanetz-Bartley琼脂上菌落形态之间的关系。在总共523株已鉴定的肠球菌中，345株（66%）形成紫色菌落，136株形成红色菌落（26%），37株形成粉色菌落（7%），5株形成奶油色菌落（1%）。肠球菌菌株的颜色、大小或菌落形态与分类学特征之间不存在相关性。
