易卒中型自发性高血压大鼠脑和心肌中超氧化物歧化酶的区域分布

Regional distribution of superoxide dismutase in the brain and myocardium of the stroke-prone spontaneously hypertensive rat.

Kimoto S, Nishida S, Funasaka K, Nakano T, Teramoto K, Tomura T T

Department of Biochemistry, Kinki University School of Medicine, Osaka-sayama, Japan.

Clin Exp Pharmacol Physiol Suppl. 1995 Dec;22(1):S160-1. doi: 10.1111/j.1440-1681.1995.tb02863.x.

Abstract

Enzyme activities and contents of manganese and copper-zinc superoxide dismutase (Mn-, Cu/Zn-SOD) and oxygen free-radical scavengers were determined in the myocardium (right, left ventricle) and brain (cerebral cortex, hippocampus) of 15 and 31 week old stroke-prone spontaneously hypertensive rats (SHRSP). 2. In 15 week old SHRSP myocardium, both Mn- and Cu/Zn-SOD activities were higher but in 31 week old SHRSP, these were lower than that in Wistar-Kyoto (WKY) rats. Further, correlation between Mn-SOD content and activity in 31 week old SHRSP myocardium showed that specific activity was lower than that in WKY. 3. In 15 and 31 week old SHRSP cerebral cortex and hippocampus, SOD content and activity showed a tendency to be lower than that in WKY. 4. These results indicate that enzymatically inactive or low-active Mn-SOD protein exists in SHRSP myocardium, and that the alteration of SOD may be one of the causative factors for the vulnerability of the myocardium and brain against O2-radicals.

摘要

测定了15周龄和31周龄易卒中型自发性高血压大鼠（SHRSP）心肌（右心室、左心室）和脑（大脑皮层、海马体）中锰和铜锌超氧化物歧化酶（Mn-SOD、Cu/Zn-SOD）的酶活性及含量以及氧自由基清除剂的水平。2. 在15周龄SHRSP的心肌中，Mn-SOD和Cu/Zn-SOD的活性均较高，但在31周龄SHRSP中，这些活性低于Wistar-Kyoto（WKY）大鼠。此外，31周龄SHRSP心肌中Mn-SOD含量与活性之间的相关性表明，其比活性低于WKY大鼠。3. 在15周龄和31周龄SHRSP的大脑皮层和海马体中，SOD的含量和活性有低于WKY大鼠的趋势。4. 这些结果表明，SHRSP心肌中存在无酶活性或低活性的Mn-SOD蛋白，并且SOD的改变可能是心肌和脑对氧自由基易感性的致病因素之一。