凝血酶和血小板衍生生长因子对自发性高血压大鼠系膜细胞培养物中内皮素生成的影响。

Effect of thrombin and PDGF on endothelin production in cultured mesangial cells derived from spontaneously hypertensive rats.

Ikeda M, Kohno M, Horio T, Yasunari K, Yokokawa K, Kano H, Minami M, Hanehira T, Fukui T, Takeda T

First Department of Internal Medicine, Osaka City University Medical School, Japan.

Clin Exp Pharmacol Physiol Suppl. 1995 Dec;22(1):S197-8. doi: 10.1111/j.1440-1681.1995.tb02879.x.

Abstract

Basal endothelin-1 (ET-1) production in mesangial cells of spontaneously hypertensive rats (SHR) was not different from that of Wistar-Kyoto (WKY) rats, although a trend toward increased ET-1 production was observed in these cells of SHR. 2. Thrombin and platelet-derived growth factor (PDGF) stimulated ET-1 production in a concentration-dependent manner in these cells of both rat strains, but thrombin- and PDGF-induced stimulation of ET-1 production were clearly greater in cells of SHR than WKY rats. 3. The protein kinase C (PKC)-activating phorbol ester, phorbol myristate acetate, stimulated ET-1 production in cells of both rat strains, but this stimulation was significantly greater in cells of SHR than in cells of WKY rats. 4. An inactive enantiomer of phorbol ester, 4alpha-PDD, had no effect on the ET-1 production in these cells of both rat strains. 5. Neither thrombin nor PDGF stimulated ET-1 production in PKC-depleted cells of both rat strains.

摘要

自发性高血压大鼠（SHR）系膜细胞中基础内皮素-1（ET-1）的产生与Wistar-Kyoto（WKY）大鼠的无异，尽管在SHR的这些细胞中观察到ET-1产生有增加的趋势。2. 凝血酶和血小板衍生生长因子（PDGF）在两种大鼠品系的这些细胞中均以浓度依赖性方式刺激ET-1产生，但凝血酶和PDGF诱导的ET-1产生刺激在SHR细胞中明显大于WKY大鼠。3. 蛋白激酶C（PKC）激活剂佛波酯，肉豆蔻酸佛波醇酯，在两种大鼠品系的细胞中均刺激ET-1产生，但这种刺激在SHR细胞中明显大于WKY大鼠细胞。4. 佛波酯的无活性对映体4α-PDD对两种大鼠品系的这些细胞中的ET-1产生无影响。5. 凝血酶和PDGF均未刺激两种大鼠品系PKC缺失细胞中的ET-1产生。