Parks S R, Bennett A M, Speight S E, Benbough J E
Biosafety Investigation Unit, Centre for Applied Microbiology and Research, Porton Down, Salisbury, UK.
J Appl Bacteriol. 1996 May;80(5):529-34. doi: 10.1111/j.1365-2672.1996.tb03252.x.
Tests described in this paper show that gelatine membrane filters used in the MD8 microbiological air sampling system collected monodispersed aerosols between 0.7 and 1.0 microns containing viable Bacillus subtilis var. niger spores, with an efficiency of 99.9995%. Gelatine membrane filters linked to the MD8 control pump system were as effective as the well established Casella slit-to-agar device for collecting some viable bacteria, nebulized under controlled experimental conditions and naturally occurring airborne micro-organisms in a pharmaceutical plant. By using a long flexible hose connection to the control pump, the head could be positioned where sampling was required in locations remote from the pump exhaust, making it suitable for microbiological monitoring in critical locations such as laminar flow stations and isolators.
本文所述测试表明,MD8微生物空气采样系统中使用的明胶膜过滤器收集了粒径在0.7至1.0微米之间含有存活的枯草芽孢杆菌黑色变种孢子的单分散气溶胶,效率达99.9995%。与MD8控制泵系统相连的明胶膜过滤器在收集一些存活细菌方面与成熟的卡塞拉缝隙到琼脂装置一样有效,这些细菌是在受控实验条件下雾化的,以及制药厂中自然存在的空气传播微生物。通过使用连接到控制泵的长柔性软管,探头可以放置在远离泵排气口的需要采样的位置,使其适用于层流站和隔离器等关键位置的微生物监测。