Giovannoni G, Heales S J, Silver N C, O'Riordan J, Miller R F, Land J M, Clark J B, Thompson E J
Department of Neuroimmunology, The National Hospital for Neurology and Neurosurgery, London, UK.
J Neurol Sci. 1997 Jan;145(1):77-81. doi: 10.1016/s0022-510x(96)00246-8.
Nitric oxide and its highly reactive derivative peroxynitrite have been implicated as non-specific inflammatory mediators of neuronal and oligodendrocyte damage and death in multiple sclerosis. In a cross-sectional study we found levels of the nitric oxide metabolites nitrate and nitrite to be raised in the serum of patients with demyelinating disease (65.6 microM (SD 32.9)), acquired immune deficiency syndrome (57.9 microM (SD 34.9)) and inflammatory neurological disease (57.5 microM (SD 31.3)), compared with normal control subjects (32.8 microM (SD 12.2)) and patients with non-inflammatory neurological disease (41.1 microM (SD 12.3), p < 0.001). Nitric oxide metabolites were raised in all clinical subtypes of multiple sclerosis, as well as in clinically isolated syndromes compatible with demyelination, and were not related to progressive disease or disability. This study provides further evidence for a role of nitric oxide in the immunopathogenesis of inflammatory diseases of the central nervous system, including multiple sclerosis.
一氧化氮及其高反应性衍生物过氧亚硝酸盐被认为是多发性硬化症中神经元和少突胶质细胞损伤及死亡的非特异性炎症介质。在一项横断面研究中，我们发现与正常对照受试者（32.8微摩尔/升（标准差12.2））和非炎性神经系统疾病患者（41.1微摩尔/升（标准差12.3），p<0.001）相比，脱髓鞘疾病患者（65.6微摩尔/升（标准差32.9））、获得性免疫缺陷综合征患者（57.9微摩尔/升（标准差34.9））和炎性神经系统疾病患者（57.5微摩尔/升（标准差31.3））血清中的一氧化氮代谢产物硝酸盐和亚硝酸盐水平升高。一氧化氮代谢产物在多发性硬化症的所有临床亚型以及与脱髓鞘相符的临床孤立综合征中均升高，且与疾病进展或残疾无关。这项研究为一氧化氮在包括多发性硬化症在内的中枢神经系统炎性疾病的免疫发病机制中发挥作用提供了进一步证据。
