Giovannoni G, Miller R F, Heales S J, Land J M, Harrison M J, Thompson E J
Department of Neuroimmunology, The Natioinal Hospital for Neurology and Neurosurgery, London, UK.
J Neurol Sci. 1998;156(1):53-8. doi: 10.1016/s0022-510x(98)00021-5.
As nitric oxide (.NO) is hypothesised to play a role in the immunopathogenesis of neurological complications associated with inflammation, we compared levels of cerebrospinal fluid (CSF) and serum .NO metabolites in 24 patients with HIV-1 infection, to those in 58 non-HIV infected patients with neurological disorders. Levels of .NO metabolites were correlated with blood-brain-barrier dysfunction. CSF and serum nitrate and nitrite levels were measured by the nitrate reductase and Griess reaction methods. The .NO metabolites, nitrate and nitrite, were raised in the CSF and serum of patients with AIDS and central nervous system complications, when compared to non-HIV infected patients with inflammatory and non-inflammatory neurological disorders (median nitrate and nitrite: CSF=18.3 microM vs. 11.1 microM vs. 7.0 microM, P<0.001, and serum=53.8 microM vs. 50.3 microM vs. 41.4 microM, P=0.04, respectively). CSF nitrate and nitrite levels correlated with the albumin quotient. This study supports the evidence that .NO is a potential mediator of blood-brain-barrier breakdown in inflammatory diseases of the central nervous system.
由于一氧化氮（.NO）被认为在与炎症相关的神经系统并发症的免疫发病机制中起作用，我们比较了24例HIV-1感染患者与58例非HIV感染的神经系统疾病患者脑脊液（CSF）和血清中.NO代谢产物的水平。.NO代谢产物的水平与血脑屏障功能障碍相关。通过硝酸还原酶法和格里斯反应法测量脑脊液和血清中的硝酸盐和亚硝酸盐水平。与非HIV感染的炎症性和非炎症性神经系统疾病患者相比，艾滋病和中枢神经系统并发症患者的脑脊液和血清中的.NO代谢产物硝酸盐和亚硝酸盐升高（中位数硝酸盐和亚硝酸盐：脑脊液=18.3微摩尔/升 vs. 11.1微摩尔/升 vs. 7.0微摩尔/升，P<0.001，血清=53.8微摩尔/升 vs. 50.3微摩尔/升 vs. 41.4微摩尔/升，P=0.04）。脑脊液硝酸盐和亚硝酸盐水平与白蛋白商数相关。本研究支持以下证据：.NO是中枢神经系统炎症性疾病中血脑屏障破坏的潜在介质。
