Marín I, Amils R, Abad J P
Centro de Biología Molecular Severo Ochoa (C.S.I.C.-U.A.M.), Universidad Autonóma de Madrid, Cantoblanco, Spain.
Gene. 1997 Mar 10;187(1):99-105. doi: 10.1016/s0378-1119(96)00731-7.
The genomic organization of Thiobacillus cuprinus, a facultative chemolithotrophic bacterium that preferentially solubilizes copper from complex ores, has been studied by Pulsed Field Gel Electrophoresis (PFGE). It has been determined that T. cuprinus has a circular chromosome of about 3.8 Mb in size as concluded by analysis of gamma-irradiated total DNA and restriction analysis. Macrorestriction patterns for several restriction enzymes have been generated. Restriction enzymes AseI, DraI, SpeI, SwaI and XbaI give a number of fragments that can be adequately resolved by PFGE and then be used for electrophoretic karyotyping and for the construction of physical maps of the chromosome. Such a map has been constructed for the endonucleases SpeI and SwaI. The localization of several heterologous and homologous genes on the physical map, including those for rRNA, has paved the way for the construction of a genetic map.
利用脉冲场凝胶电泳（PFGE）技术对嗜铜硫杆菌（Thiobacillus cuprinus）的基因组结构进行了研究。嗜铜硫杆菌是一种兼性化能自养细菌，能优先从复合矿石中溶解铜。通过对γ射线辐照的总DNA进行分析和限制性分析，已确定嗜铜硫杆菌拥有一条大小约为3.8 Mb的环状染色体。已生成了几种限制性内切酶的宏观限制性图谱。限制性内切酶AseI、DraI、SpeI、SwaI和XbaI产生的多个片段可通过PFGE得到充分分离，进而用于电泳核型分析和构建染色体物理图谱。已构建了针对内切酶SpeI和SwaI的此类图谱。包括rRNA基因在内的几个异源和同源基因在物理图谱上的定位，为构建遗传图谱铺平了道路。
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