嗜酸化能自养细菌氧化亚铁硫杆菌ATCC 21834的基因组结构
Genomic organization of the acidophilic chemolithoautotrophic bacterium Thiobacillus ferrooxidans ATCC 21834.
作者信息
Irazabal N, Marín I, Amils R
机构信息
Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Universidad Autónoma de Madrid, Spain.
出版信息
J Bacteriol. 1997 Mar;179(6):1946-50. doi: 10.1128/jb.179.6.1946-1950.1997.
Abstract
The genomic organization of the acidophilic chemolithoautotrophic bacterium Thiobacillus ferrooxidans ATCC 21834 has been studied by pulsed-field gel electrophoresis (PFGE). Analysis of its intact DNA, as well as the restriction patterns obtained with several endonucleases, allowed the characterization of one circular chromosome of 2.9 Mb and one plasmid of 8.6 kb. The first complete and highly resolved physical map (86 restriction sites) of the chromosome of an acidophilic obligate chemolithoautotrophic bacterium has been constructed by using endonucleases PmeI, SwaI, XbaI, and SpeI. The rRNA and str operons have been located on the chromosomal physical map.
摘要
利用脉冲场凝胶电泳（PFGE）对嗜酸化能自养细菌氧化亚铁硫杆菌ATCC 21834的基因组结构进行了研究。对其完整DNA以及用几种核酸内切酶获得的限制性图谱进行分析，确定了一条2.9 Mb的环状染色体和一个8.6 kb的质粒。通过使用核酸内切酶PmeI、SwaI、XbaI和SpeI，构建了嗜酸专性化能自养细菌染色体的首张完整且分辨率高的物理图谱（86个限制性位点）。rRNA和str操纵子已定位在染色体物理图谱上。
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