Kumar S, Harvey K F, Kinoshita M, Copeland N G, Noda M, Jenkins N A
The Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Rundle Mall, Adelaide, South Australia, 5000, Australia.
Genomics. 1997 Mar 15;40(3):435-43. doi: 10.1006/geno.1996.4582.
The Nedd4 gene was initially identified by a subtraction cloning approach as a highly expressed transcript in the mouse embryonic brain. Cloning of the Nedd4 cDNA indicated that it can encode a protein of approximately 103 kDa, consisting of a Ca2+ and phospholipid binding domain, three putative protein-protein interaction domains (the WW domains), and a carboxyl-terminus region similar to the ubiquitin-protein ligase domain (hect domain). In mouse embryos, the expression of Nedd4 in the central nervous system is highest during neurogenesis and decreases as development progresses. In addition to the central nervous system, the expression of Nedd4 is detected in various embryonic tissues and persists in most adult tissues. Using an antibody raised against a fusion protein, we show that Nedd4 protein is localized to the cellular cytoplasm. We have mapped the mouse Nedd4 gene to chromosome 9 using an interspecific backcross panel. Nedd4 maps to a previously defined homologous region between human and mouse chromosomes and thus provides additional information regarding interspecies comparative mapping.
Nedd4基因最初是通过消减克隆方法鉴定出来的，它是小鼠胚胎脑中高表达的转录本。Nedd4 cDNA的克隆表明，它可以编码一种约103 kDa的蛋白质，该蛋白质由一个Ca2+和磷脂结合结构域、三个假定的蛋白质-蛋白质相互作用结构域（WW结构域）以及一个与泛素-蛋白质连接酶结构域（HECT结构域）相似的羧基末端区域组成。在小鼠胚胎中，Nedd4在中枢神经系统中的表达在神经发生过程中最高，并随着发育进程而降低。除中枢神经系统外，Nedd4在各种胚胎组织中均有表达，并在大多数成年组织中持续存在。使用针对融合蛋白产生的抗体，我们发现Nedd4蛋白定位于细胞质中。我们利用种间回交群体将小鼠Nedd4基因定位到9号染色体上。Nedd4定位于人类和小鼠染色体之间先前定义的同源区域，因此提供了有关种间比较图谱的额外信息。
