Turgeon B, Saba-El-Leil M K, Meloche S
Research Centre, Centre hospitalier de l'Université de Montréal, Hotel-Dieu Campus, 3850 Saint Urbain Street, Montreal, Quebec, Canada.
Biochem J. 2000 Feb 15;346 Pt 1(Pt 1):169-75.
MAP (mitogen-activated protein) kinases are a family of serine/threonine kinases that have a pivotal role in signal transduction. Here we report the cloning and characterization of a mouse homologue of extracellular-signal-regulated protein kinase (ERK)3. The mouse Erk3 cDNA encodes a predicted protein of 720 residues, which displays 94% identity with human ERK3. Transcription and translation of this cDNA in vitro generates a 100 kDa protein similar to the human gene product ERK3. Immunoblot analysis with an antibody raised against a unique sequence of ERK3 also recognizes a 100 kDa protein in mouse tissues. A single transcript of Erk3 was detected in every adult mouse tissue examined, with the highest expression being found in the brain. Interestingly, expression of Erk3 mRNA is acutely regulated during mouse development, with a peak of expression observed at embryonic day 11. The mouse Erk3 gene was mapped to a single locus on central mouse chromosome 9, adjacent to the dilute mutation locus and in a region syntenic to human chromosome 15q21. Finally, we provide several lines of evidence to support the existence of a unique Erk3 gene product of 100 kDa in mammalian cells.
丝裂原活化蛋白（MAP）激酶是丝氨酸/苏氨酸激酶家族，在信号转导中起关键作用。在此，我们报告细胞外信号调节蛋白激酶（ERK）3的小鼠同源物的克隆与特性。小鼠Erk3 cDNA编码一个预测有720个残基的蛋白质，与人类ERK3有94%的同一性。该cDNA在体外转录和翻译产生一个100 kDa的蛋白质，类似于人类基因产物ERK3。用针对ERK3独特序列产生的抗体进行免疫印迹分析，也能识别小鼠组织中的一个100 kDa蛋白质。在检测的每个成年小鼠组织中都检测到了单一的Erk3转录本，在脑中表达最高。有趣的是，Erk3 mRNA的表达在小鼠发育过程中受到急性调节，在胚胎第11天观察到表达峰值。小鼠Erk3基因定位于小鼠中央9号染色体上的一个单一基因座，与稀释突变基因座相邻，且位于与人类15号染色体q21同源的区域。最后，我们提供了几条证据来支持哺乳动物细胞中存在一种独特的100 kDa的Erk3基因产物。
