Carrette O, Mizon C, Sautière P, Sesboüé R, Mizon J
Laboratoire de Biochimie, Faculté de Pharmacie, Lille, France.
Biochim Biophys Acta. 1997 Mar 7;1338(1):21-30. doi: 10.1016/s0167-4838(96)00184-7.
With the view of investigating the metabolism of inter-alpha-inhibitor, a plasma serine-proteinase inhibitor, in an animal model of inflammatory syndrome, we isolated inter-alpha-inhibitor from pig plasma. A high yield was obtained (140 mg/liter) with a two-step procedure: anion-exchange chromatography followed by affinity chromatography on heparin-Sepharose. In contrast to bovine inter-alpha-inhibitor was highly similar to human inter-alpha-inhibitor: its heavy chains are homologous to the human H1 and H2 heavy chains, as shown by chromatographic and electrophoretic properties, cross-immunoreactivity and N-terminal sequencing. Pig may therefore represent a good animal model to study inter-alpha-inhibitor metabolism and elucidate its physiological role.
为了研究血浆丝氨酸蛋白酶抑制剂α-间抑制因子在炎症综合征动物模型中的代谢情况，我们从猪血浆中分离出了α-间抑制因子。通过两步法获得了高产率（140毫克/升）：首先进行阴离子交换色谱，然后在肝素琼脂糖凝胶上进行亲和色谱。与牛α-间抑制因子不同，猪α-间抑制因子与人类α-间抑制因子高度相似：通过色谱和电泳特性、交叉免疫反应性和N端测序表明，其重链与人类H1和H2重链同源。因此，猪可能是研究α-间抑制因子代谢并阐明其生理作用的良好动物模型。
