肌球蛋白重链mRNA在固定化骨骼肌中转变为更快的亚型：一项定量PCR研究。
Myosin heavy chain mRNA transform to faster isoforms in immobilized skeletal muscle: a quantitative PCR study.
作者信息
Jänkälä H, Harjola V P, Petersen N E, Härkönen M
机构信息
Department of Clinical Chemistry, University of Helsinki, Finland.
出版信息
J Appl Physiol (1985). 1997 Mar;82(3):977-82. doi: 10.1152/jappl.1997.82.3.977.
A quantitative polymerase chain reaction (PCR) method was used to measure the quantities of type I, IIa, IIx, and IIb myosin heavy chain (MHC) mRNA in total RNA preparations of the soleus, gastrocnemius, and plantaris muscles of normal and hindlimb-immobilized rats. Type IIx and even type IIb MHC mRNA were demonstrated at extremely low levels in normal soleus, 2.1 +/- 0.4 x 10(5) and 5.0 +/- 0.2 x 10(5) molecules of mRNA per microgram total RNA, respectively. Immobilization for 1 wk significantly altered the gene expression of MHC isoforms. In soleus, both type IIx and IIb MHC genes became significantly upregulated, 24-fold (P < 0.005) and 2.6-fold (P < 0.05), respectively. In gastrocnemius, the level of type IIa MHC mRNA decreased by 51% (P < 0.01) and the level of type IIx MHC mRNA increased by 140% (P < 0.05). In plantaris, the level of type IIa MHC mRNA decreased by 58% (P < 0.005). In conclusion, immobilization changed the MHC mRNA profile in three different types of skeletal muscle toward faster isoforms. The quantitative results permit reliable evaluation of changes in mRNA levels.
采用定量聚合酶链反应（PCR）方法，检测正常大鼠和后肢固定大鼠比目鱼肌、腓肠肌和跖肌总RNA制剂中I型、IIa型、IIx型和IIb型肌球蛋白重链（MHC）mRNA的含量。在正常比目鱼肌中，IIx型甚至IIb型MHC mRNA水平极低，分别为每微克总RNA 2.1±0.4×10⁵和5.0±0.2×10⁵个mRNA分子。固定1周显著改变了MHC亚型的基因表达。在比目鱼肌中，IIx型和IIb型MHC基因均显著上调，分别上调24倍（P<0.005）和2.6倍（P<0.05）。在腓肠肌中，IIa型MHC mRNA水平下降51%（P<0.01），IIx型MHC mRNA水平上升140%（P<0.05）。在跖肌中，IIa型MHC mRNA水平下降58%（P<0.005）。总之，固定使三种不同类型骨骼肌的MHC mRNA谱向更快的亚型转变。定量结果有助于可靠评估mRNA水平的变化。
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