Woo E, Tingey D P, Mackenzie G, Hooper P
Ivey Institute of Ophthalmology, Victoria Hospital, University of Western Ontario, London, Canada.
J Glaucoma. 1997 Feb;6(1):33-6.
The purpose of this study was to determine the long-term stability of the antiproliferative effect of mitomycin-C (MMC) following reconstitution.
Identical MMC preparations were reconstituted from the crystalline form and then stored at room temperature or 4 degrees C. Cultured human Tenon's fibroblasts were exposed to MMC (0.5 mg/ml) for 2.5 min at 0, 3, 7, 10, 14, 21, 28, 35, and 42 days following reconstitution. After removal of the drug, fibroblast proliferation was measured by tritiated thymidine uptake.
The percentage of inhibition was maintained at > 85% for both the room temperature and the 4 degrees C groups, and this inhibition persisted for the duration of the experiment. There was no statistically significant difference in the results between the storage temperatures.
These data suggest that MMC retains its antiproliferative effect for at least 6 weeks following reconstitution and that this effect is not changed by storage temperature. Cost savings may be realized by storing MMC in unit-dose reconstituted aliquots for these longer periods, providing sterility can be assured.
本研究的目的是确定丝裂霉素-C（MMC）复溶后抗增殖作用的长期稳定性。
将相同的MMC制剂从结晶形式复溶，然后在室温或4℃下储存。在复溶后的0、3、7、10、14、21、28、35和42天，将培养的人Tenon成纤维细胞暴露于MMC（0.5mg/ml）中2.5分钟。去除药物后，通过氚标记胸腺嘧啶核苷摄取来测量成纤维细胞增殖。
室温组和4℃组的抑制率均维持在>85%，并且这种抑制在实验期间持续存在。储存温度之间的结果没有统计学上的显著差异。
这些数据表明，MMC复溶后至少6周仍保留其抗增殖作用，并且这种作用不受储存温度的影响。如果能确保无菌，将MMC以单位剂量复溶分装的形式储存更长时间可能会节省成本。
