Rao K M, Hatchell D L, Cohen H J, De La Paz M A
Department of Geriatric Research, Education and Clinical Center, Veterans Affairs Medical Center, Duke University Medical Center, Durham, North Carolina, USA.
Am J Med Sci. 1997 Mar;313(3):131-7. doi: 10.1097/00000441-199703000-00002.
Adhesion molecule expression on peripheral blood leukocytes from diabetic patients with severe retinopathy and age-matched control subjects was assessed. Expression of CD11b, CD18, and L-selectin was measured on granulocytes and lymphocytes in whole blood within 1 hour of blood collection. Adhesion molecule expression was determined at 4 degrees C, 37 degrees C, and after stimulation with one of the chemotactic peptides, N-formyl-methionyl-leucyl-phenyl-alanine or beta-phorbol 12-myristate 13-acetate. There were no differences between diabetics and controls in CD11b expression in neutrophils at 4 degrees C, 37 degrees C, or after N-formyl-methionyl-leucyl-phenylalanine stimulation. However, during stimulation with beta-phorbol 12-myristate 13-acetate, the increase in CD11b expression in neutrophils from patients with diabetes was significantly less than in controls. In neutrophils, there was no difference between the control and diabetic participants in CD18 expression at 4 degrees C, but after warming the cells to 37 degrees C, the expression was significantly higher in patients with diabetes. The difference became even more apparent after N-formyl-methionyl-leucyl-phenyl-alanine stimulation. The increase in CD18 expression after beta-phorbol 12-myristate 13-acetate stimulation of neutrophils was similar in control and diabetic participants. There was no difference in L-selectin expression in neutrophils under any conditions. There was no difference in adhesion molecule expression on lymphocytes under similar conditions. In summary, these observations indicate that integrin expression of neutrophils from patients with diabetes and retinopathy is altered after stimulation with neutrophil-activating agents. The changes were integrin-, stimulus-, and cell-specific, which suggests that the signal transduction mechanisms may be altered in diabetic neutrophils. These alterations may be responsible for abnormal leukocyte/endothelial interactions and microvascular complications in diabetic retinopathy.
评估了患有严重视网膜病变的糖尿病患者及年龄匹配的对照受试者外周血白细胞上黏附分子的表达。在采血后1小时内，测定全血中粒细胞和淋巴细胞上CD11b、CD18和L-选择素的表达。在4℃、37℃以及用趋化肽N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸或佛波酯12-肉豆蔻酸酯13-乙酸酯刺激后，测定黏附分子的表达。在4℃、37℃或经N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸刺激后，糖尿病患者和对照者中性粒细胞中CD11b的表达没有差异。然而，在用佛波酯12-肉豆蔻酸酯13-乙酸酯刺激期间，糖尿病患者中性粒细胞中CD11b表达的增加明显少于对照者。在中性粒细胞中，4℃时对照者和糖尿病参与者的CD18表达没有差异，但将细胞加热至37℃后，糖尿病患者的表达明显更高。在N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸刺激后，这种差异变得更加明显。佛波酯12-肉豆蔻酸酯13-乙酸酯刺激中性粒细胞后，对照者和糖尿病参与者中CD18表达的增加相似。在任何条件下，中性粒细胞中L-选择素的表达没有差异。在相似条件下，淋巴细胞上黏附分子的表达没有差异。总之，这些观察结果表明，糖尿病和视网膜病变患者中性粒细胞的整合素表达在用中性粒细胞激活剂刺激后发生了改变。这些变化是整合素、刺激物和细胞特异性的，这表明糖尿病中性粒细胞中的信号转导机制可能发生了改变。这些改变可能是糖尿病视网膜病变中白细胞/内皮细胞异常相互作用和微血管并发症的原因。
