Szarka R J, Wang N, Gordon L, Nation P N, Smith R H
Alberta Research Council, Carbohydrate Chemistry, Edmonton, Canada.
J Immunol Methods. 1997 Mar 10;202(1):49-57. doi: 10.1016/s0022-1759(96)00236-0.
This study examines the intranasal instillation of lipopolysaccharide (LPS) into BALB/c mice causing acute pulmonary damage, due to neutrophil infiltration and sepsis. A dose response with LPS showed that an intranasal instillation of 167 microg/ml (10 microg/mouse) caused acute lung injury within 2-4 h and reached maximal damage at 24-48 h. We found the method of LPS administration for induction of acute pulmonary damage to be crucial. After 24 h post-LPS injection, a comparison showed a substantial increase in pulmonary damage with intranasal instillation of LPS. As for intravenous injection, it showed a baseline effect. This study indicates that LPS administered intranasally causes acute pulmonary damage, whereas with intravenous and intraperitoneal endotoxin administration a tissue-specific or similar degree of pulmonary injury may not develop.
本研究探讨了通过向BALB/c小鼠鼻内滴注脂多糖（LPS）来引发急性肺损伤，这是由于中性粒细胞浸润和败血症所致。LPS的剂量反应表明，鼻内滴注167微克/毫升（10微克/小鼠）可在2至4小时内导致急性肺损伤，并在24至48小时达到最大损伤程度。我们发现LPS给药方法对于诱导急性肺损伤至关重要。LPS注射后24小时，比较结果显示鼻内滴注LPS会使肺损伤显著增加。至于静脉注射，它显示出基线效应。本研究表明，鼻内给予LPS会导致急性肺损伤，而静脉内和腹腔内给予内毒素可能不会引发组织特异性或类似程度的肺损伤。
