Baur A S, Sass G, Laffert B, Willbold D, Cheng-Mayer C, Peterlin B M
Institut für Klinische und Molekulare Virologie, Universität Erlangen/Nürnberg, Erlangen, Federal Republic of Germany.
Immunity. 1997 Mar;6(3):283-91. doi: 10.1016/s1074-7613(00)80331-3.
The Nef protein of human and primate lentiviruses is a key factor in HIV/SIV pathogenesis. Here we report that Nef associates with two different kinases, forming a multiprotein complex at the far N-terminus of the viral protein. One of the kinases was identified as Lck, whereas the second protein was found to be a serine kinase that phosphorylated Nef and Lck in vitro and could be discriminated from the serine kinase identified previously. The Nef-associated kinase complex (NAKC) was demonstrated in COS cells, in HIV-infected cells, and in vitro using recombinant Lck and Nef proteins. Deletion of a short amphipathic alpha-helix in the N-terminus, which was found to be conserved in all Nef proteins, inhibited association of the NAKC and significantly reduced virion infectivity.
人类和灵长类慢病毒的Nef蛋白是HIV/SIV发病机制中的关键因素。在此我们报告，Nef与两种不同的激酶相关联，在病毒蛋白的远N端形成一个多蛋白复合物。其中一种激酶被鉴定为Lck，而第二种蛋白是一种丝氨酸激酶，它在体外可使Nef和Lck磷酸化，并且可与先前鉴定的丝氨酸激酶区分开来。Nef相关激酶复合物（NAKC）在COS细胞、HIV感染的细胞中以及使用重组Lck和Nef蛋白在体外得到了证实。在N端缺失一个短的两亲性α螺旋（该螺旋在所有Nef蛋白中都保守），会抑制NAKC的关联并显著降低病毒体的感染性。
