长期使用抗抑郁药、维拉帕米或锂进行治疗会抑制血清素诱导的单个C6大鼠胶质瘤细胞内的钙反应。

Chronic treatment with antidepressants, verapamil, or lithium inhibits the serotonin-induced intracellular calcium response in individual C6 rat glioma cells.

作者信息

Yamaji T, Kagaya A, Uchitomi Y, Yokota N, Yamawaki S

机构信息

Department of Psychiatry and Neurosciences, Hiroshima University School of Medicine, Japan.

出版信息

Life Sci. 1997;60(11):817-23. doi: 10.1016/s0024-3205(97)00010-6.

DOI:10.1016/s0024-3205(97)00010-6

PMID:9076320

Abstract

The effects of chronic treatment with antidepressants, verapamil, or lithium on serotonin (5-HT)-induced Ca2+ increase were investigated in single C6BU-1 glioma cells with digital imaging microscopy. Clomipramine and citalopram, at a concentration of 100 nM, decreased the peak values of 5-HT-induced [Ca2+]i changes. Verapamil (100 nM), a calcium antagonist, and lithium (1 mM) also inhibited the peak amplitudes in the same way. The present findings suggest that chronic treatment with antidepressants, verapamil, or lithium, at therapeutic concentrations, have the common action of inhibiting 5-HT-mediated [Ca2+]i increase.

摘要

采用数字成像显微镜技术，在单个C6BU-1胶质瘤细胞中研究了抗抑郁药、维拉帕米或锂盐长期治疗对5-羟色胺（5-HT）诱导的钙离子增加的影响。100 nM浓度的氯米帕明和西酞普兰降低了5-HT诱导的细胞内钙离子浓度（[Ca2+]i）变化的峰值。钙拮抗剂维拉帕米（100 nM）和锂盐（1 mM）也以同样的方式抑制了峰值幅度。目前的研究结果表明，治疗浓度的抗抑郁药、维拉帕米或锂盐长期治疗具有共同作用，即抑制5-HT介导的[Ca2+]i增加。