Paz A, Lindner A, Zisman A, Siegel Y
Urology Department, Assaf Harofeh Medical Center, Zerifin, Israel.
Eur Urol. 1997;31(2):209-15. doi: 10.1159/000474452.
We intended to identify structural changes in the hormone-binding domain and C-terminal region of the androgen receptor (AR) gene in carcinoma of prostate specimens.
Twelve prostate cancer specimens from prostate resections were analyzed for the presence of mutations in the E-H exons and part of the 3'-untranslated region of the AR cDNA, using RT-PCR and direct sequencing.
A polymorphic sequence spanning 17 nucleotides was identified in the 3'-untranslated region of the AR cDNA from one prostate carcinoma tissue, suggesting the presence of a somatic mutation. No mutations were identified in the coding sequence of the hormone-binding domain of the AR cDNA.
These results suggest that AR mutations are probably uncommon molecular events in the early stages of prostate cancer.
我们旨在鉴定前列腺癌标本中雄激素受体（AR）基因的激素结合域和C端区域的结构变化。
使用逆转录聚合酶链反应（RT-PCR）和直接测序法，对12例前列腺切除术后的前列腺癌标本进行分析，以检测AR cDNA的E-H外显子和部分3'-非翻译区中是否存在突变。
在一个前列腺癌组织的AR cDNA的3'-非翻译区中鉴定出一个跨越17个核苷酸的多态性序列，提示存在体细胞突变。在AR cDNA激素结合域的编码序列中未鉴定出突变。
这些结果表明，AR突变可能是前列腺癌早期阶段不常见的分子事件。
