对已选择或未选择对噻嘧啶、左旋咪唑和伊维菌素具有抗性的齿状食道口线虫品系进行体外特性分析。

In vitro characterization of lines of Oesophagostomum dentatum selected or not selected for resistance to pyrantel, levamisole and ivermectin.

作者信息

Várady M, Bjørn H, Craven J, Nansen P

机构信息

Danish Centre for Experimental Parasitology, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.

出版信息

Int J Parasitol. 1997 Jan;27(1):77-81. doi: 10.1016/s0020-7519(96)00159-2.

DOI:10.1016/s0020-7519(96)00159-2

PMID:9076532

Abstract

Lines of Oesophagostomum dentatum artificially selected or not selected for resistance to pyrantel, levamisole and ivermectin were used in this study. From the 10th generation of selection eggs were collected from each line and subjected to an in vitro larval development assay (LDA) and an egg hatch assay (EHPA). Significant differences were observed between an unselected line of O. dentatum and the lines selected for resistance to levamisole or pyrantel in both assays. The LDA was more sensitive than EHPA in detecting anthelmintic resistance in O. dentatum. The results obtained from the LDA confirmed side-resistance between levamisole and morantel/pyrantel. The in vitro tests failed to show significant differences between ivermectin-sensitive and resistant lines.

摘要

本研究使用了对噻嘧啶、左旋咪唑和伊维菌素具有抗性或未经过人工选择抗性的齿状食道口线虫品系。从第10代选择开始，从每个品系收集虫卵，并进行体外幼虫发育试验（LDA）和虫卵孵化试验（EHPA）。在这两种试验中，未选择的齿状食道口线虫品系与选择的对左旋咪唑或噻嘧啶具有抗性的品系之间观察到显著差异。在检测齿状食道口线虫的抗蠕虫药抗性方面，LDA比EHPA更敏感。LDA获得的结果证实了左旋咪唑与莫仑太尔/噻嘧啶之间存在交叉抗性。体外试验未能显示伊维菌素敏感和抗性品系之间的显著差异。