紫花苜蓿种子中铁蛋白的纯化与特性分析

Purification and characterization of ferritin from alfalfa seeds.

作者信息

Barceló F, Miralles F, Otero Areán C

机构信息

Departamento de Biología Fundamental y Ciencias de la Salud, Universidad de las Islas Baleares, Palma de Mallorca, Spain.

出版信息

J Inorg Biochem. 1997 Apr;66(1):23-7. doi: 10.1016/s0162-0134(96)00155-9.

DOI:10.1016/s0162-0134(96)00155-9

PMID:9076971

Abstract

Ferritin from alfalfa (Medicago sativa) seeds was isolated, purified, and characterized. The apparent molecular mass of the native protein was found to be 560 kDa. Electrophoresis in denaturing gradient polyacrylamide-SDS gels revealed subunits of 28-26.5 kDa. The average iron cores were 4 nm in diameter and contained about 1400 iron atoms, with an iron-to-phosphorus ratio of 4:1. N-terminal amino acid sequencing of the 28 kDa subunit revealed close homology with other plant proteins. Immunochemical analysis using polyclonal antibodies raised against pea-seed ferritin has confirmed, in agreement with previous reports, that plant proteins share common epitopes.

摘要

从紫花苜蓿（Medicago sativa）种子中分离、纯化并鉴定了铁蛋白。发现天然蛋白的表观分子量为560 kDa。在变性梯度聚丙烯酰胺-SDS凝胶中电泳显示亚基分子量为28 - 26.5 kDa。平均铁核直径为4 nm，含有约1400个铁原子，铁与磷的比例为4:1。对28 kDa亚基进行N端氨基酸测序，结果显示其与其他植物蛋白具有高度同源性。与之前的报道一致，使用针对豌豆种子铁蛋白产生的多克隆抗体进行免疫化学分析证实，植物蛋白具有共同的表位。