Barceló F, Miralles F, Otero Areán C
Departamento de Biología Fundamental y Ciencias de la Salud, Universidad de las Islas Baleares, Palma de Mallorca, Spain.
J Inorg Biochem. 1997 Apr;66(1):23-7. doi: 10.1016/s0162-0134(96)00155-9.
Ferritin from alfalfa (Medicago sativa) seeds was isolated, purified, and characterized. The apparent molecular mass of the native protein was found to be 560 kDa. Electrophoresis in denaturing gradient polyacrylamide-SDS gels revealed subunits of 28-26.5 kDa. The average iron cores were 4 nm in diameter and contained about 1400 iron atoms, with an iron-to-phosphorus ratio of 4:1. N-terminal amino acid sequencing of the 28 kDa subunit revealed close homology with other plant proteins. Immunochemical analysis using polyclonal antibodies raised against pea-seed ferritin has confirmed, in agreement with previous reports, that plant proteins share common epitopes.
从紫花苜蓿（Medicago sativa）种子中分离、纯化并鉴定了铁蛋白。发现天然蛋白的表观分子量为560 kDa。在变性梯度聚丙烯酰胺-SDS凝胶中电泳显示亚基分子量为28 - 26.5 kDa。平均铁核直径为4 nm，含有约1400个铁原子，铁与磷的比例为4:1。对28 kDa亚基进行N端氨基酸测序，结果显示其与其他植物蛋白具有高度同源性。与之前的报道一致，使用针对豌豆种子铁蛋白产生的多克隆抗体进行免疫化学分析证实，植物蛋白具有共同的表位。
