Bhakdi S, Bhakdi-Lehnen B, Bjerrum O J
Biochim Biophys Acta. 1977 Oct 3;470(1):35-44. doi: 10.1016/0005-2736(77)90059-1.
Charge-shift electrophoresis has been suggested as a simple and novel method for differentiating between emphiphilic and hydrophilic proteins (Helenius, A. and Simons, K. (1977) Proc. Natl. Acad. Sci. U.S. 74, 529-532.) This communication reports on the combination of charge-shift electrophoresis with second dimensional quantitative immunoelectrophoresis, and on a two-dimensional modification of the charge-shift electrophoresis technique. From results obtained with unfractionated human plasma proteins and human erythrocyte membrane proteins we conclude that these modifications reliably permit detection of amphiphilic proteins and peptides in complex mixtures.
电荷转移电泳已被认为是一种区分亲脂性和亲水性蛋白质的简单而新颖的方法（海伦纽斯，A.和西蒙斯，K.（1977年）《美国国家科学院院刊》74卷，529 - 532页）。本通讯报道了电荷转移电泳与二维定量免疫电泳的结合，以及电荷转移电泳技术的二维改进。从对未分级的人血浆蛋白和人红细胞膜蛋白的实验结果中我们得出结论，这些改进能够可靠地检测复杂混合物中的两亲性蛋白质和肽。
