由含植烷酸的脂质制成的脂质双分子层膜的特性。

Properties of lipid bilayer membranes made from lipids containing phytanic acid.

作者信息

Janko K, Benz R

出版信息

Biochim Biophys Acta. 1977 Oct 3;470(1):8-16. doi: 10.1016/0005-2736(77)90057-8.

Abstract

Besides the preparation of phytanic acid (3,7,11,15-tetramethylhexadecylic acid) according to the Dumas-Stass reaction, the synthesis of four different lipids containing phytanic acid residues is described. Diphytanoyl phosphatidylcholine was synthesized beginning from glycerylphosphorylcholine, whereas the other lipids, diphytanoyl phosphatidylethanolamine, diphytanoyl phosphatidylserine and monophytanoyl glyceride were prepared by total synthesis. Some properties of lipid bilayer membranes made from the lipids containing phytanic acid were investigated. The specific capacity of these membranes was measured. Its value of approximately 400 nF cm-2 was found to be similar to the value of membranes from lipids with unbranched fatty acid residues. Charge pulse experiments were performed using dipicrylamine as a molecular probe of membrane structure. The results were discussed on the basis of a higher viscosity of the membranes from lipids containing phytanic acid residues compared with unbranched fatty acid residues.

摘要

除了根据杜马斯 - 斯塔斯反应制备植烷酸（3,7,11,15 - 四甲基十六烷酸）外，还描述了四种含有植烷酸残基的不同脂质的合成。二植烷酰磷脂酰胆碱从甘油磷酰胆碱开始合成，而其他脂质，二植烷酰磷脂酰乙醇胺、二植烷酰磷脂酰丝氨酸和单植烷酰甘油则通过全合成制备。研究了由含植烷酸的脂质制成的脂质双分子层膜的一些性质。测量了这些膜的比电容。发现其约400 nF cm⁻²的值与具有直链脂肪酸残基的脂质膜的值相似。使用二苦胺作为膜结构的分子探针进行了电荷脉冲实验。基于含植烷酸残基的脂质膜与直链脂肪酸残基脂质膜相比具有更高的粘度对结果进行了讨论。