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[研究组织培养中纤维蛋白溶解激活剂释放的新方法]

[New method of studying the release of fibrinolysis activators in tissue cultures].

作者信息

Pandolfi M, Astedt B

出版信息

Biull Eksp Biol Med. 1977 Aug;84(8):250-3.

PMID:907833
Abstract

Tissue cultivation in the presence of standardized fibrin clot containing plasminogen permitted to reveal and to study quantitatively the relase of the fibrinolysis activators into the medium (by the amount of the fibrin-fibrinogen degradation products). A possibility of in vitro study of the regulation of fibrinolysis activators release by the tissues was offered by the method described.

摘要

在含有纤溶酶原的标准化纤维蛋白凝块存在的情况下进行组织培养,使得能够揭示并定量研究纤溶酶原激活剂释放到培养基中的情况(通过纤维蛋白-纤维蛋白原降解产物的量)。所描述的方法为体外研究组织对纤溶酶原激活剂释放的调节提供了可能性。

相似文献

1
[New method of studying the release of fibrinolysis activators in tissue cultures].[研究组织培养中纤维蛋白溶解激活剂释放的新方法]
Biull Eksp Biol Med. 1977 Aug;84(8):250-3.
2
Comparison of the relative fibrinogenolytic, fibrinolytic and thrombolytic properties of tissue plasminogen activator and urokinase in vitro.组织型纤溶酶原激活剂与尿激酶在体外的相对纤维蛋白溶解、纤溶及溶栓特性比较
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Human urokinase and porcine tissue plasminogen activator. A comparative study of the mechanism of fibrinolysis, and the effect of natural proteinase inhibitors and omega-aminocarboxylic acids.
Dan Med Bull. 1977 Oct;24(5):189-206.
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Fluorogenic fibrinogen and fibrin facilitate macromolecular assembly and dynamic assay of picomolar levels of plasminogen activators under well mixed conditions.荧光素标记的纤维蛋白原和纤维蛋白有助于在充分混合的条件下进行大分子组装以及对皮摩尔水平的纤溶酶原激活剂进行动态检测。
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Absence of synergism between tissue-type plasminogen activator (t-PA), single-chain urokinase-type plasminogen activator (scu-PA) and urokinase on clot lysis in a plasma milieu in vitro.组织型纤溶酶原激活剂(t-PA)、单链尿激酶型纤溶酶原激活剂(scu-PA)和尿激酶在体外血浆环境中对血凝块溶解不存在协同作用。
Thromb Haemost. 1986 Aug 20;56(1):35-9.
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[Thrombolysis using tissue-type plasminogen activator and pro-urokinase: synergism; mutants and hybrids].[使用组织型纤溶酶原激活剂和尿激酶原进行溶栓:协同作用;突变体和杂交体]
Sangre (Barc). 1989 Aug;34(4):297-9.
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Tissue plasminogen activator (tPA) inhibits plasmin degradation of fibrin. A mechanism that slows tPA-mediated fibrinolysis but does not require alpha 2-antiplasmin or leakage of intrinsic plasminogen.组织型纤溶酶原激活剂(tPA)抑制纤溶酶对纤维蛋白的降解。该机制可减缓tPA介导的纤维蛋白溶解,但不需要α2-抗纤溶酶或内源性纤溶酶原的泄漏。
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Effects of pro-urokinase and urokinase on the fibrinolytic system in man.
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