Hakugawa J, Bae S J, Vinuesa M A, Tanaka Y, Katayama I
Department of Dermatology, Nagasaki University School of Medicine.
Arerugi. 1997 Jan;46(1):42-8.
In this study we investigated the effect of LTB4 antagonist on eosinophil infiltration in skin and gut late phase response (LPR) in OVA-sensitized BALB/c mice. The eosinophil infiltrations to skin and gut induced by skin and oral challenge reached a peak at 12 h and 6 h after the challenge, respectively. Intraperitoneal administration of LTB4 antagonist (ONO-4057) before the challenge significantly inhibited eosinophil infiltrations to the skin and gut by 53.3% and 73.7%, respectively (p < 0.05). Next, we investigated the effect to that by PAF antagonist (ONO-6240) and anti-IL-5 mAb in the skin system. OVA-induced eosinophil infiltration at 12 h after intracutaneous challenge was significantly inhibited by peritoneal administration of anti-IL-5 mAb before the challenge by 89.6% (p < 0.05), but not by that of PAF antagonist. Our results demonstrated the inhibitory effect of LTB4 antagonist on eosinophil infiltration in skin and gut LPR, suggesting the potency of LTB4 antagonist for treatment of skin lesion and food allergy in atopic dermatitis considered to be associated with LPR.
在本研究中，我们调查了白三烯B4（LTB4）拮抗剂对卵清蛋白（OVA）致敏的BALB/c小鼠皮肤和肠道晚期反应（LPR）中嗜酸性粒细胞浸润的影响。皮肤和口服激发诱导的皮肤和肠道嗜酸性粒细胞浸润分别在激发后12小时和6小时达到峰值。在激发前腹腔注射LTB4拮抗剂（ONO-4057）可分别显著抑制皮肤和肠道嗜酸性粒细胞浸润53.3%和73.7%（p<0.05）。接下来，我们在皮肤系统中研究了血小板活化因子（PAF）拮抗剂（ONO-6240）和抗白细胞介素-5单克隆抗体（anti-IL-5 mAb）对此的影响。在皮内激发前腹腔注射抗IL-5 mAb可显著抑制OVA诱导的激发后12小时皮肤嗜酸性粒细胞浸润89.6%（p<0.05），但PAF拮抗剂无此作用。我们的结果证明了LTB4拮抗剂对皮肤和肠道LPR中嗜酸性粒细胞浸润的抑制作用，提示LTB4拮抗剂在治疗被认为与LPR相关的特应性皮炎皮肤病变和食物过敏方面的潜力。
