Findlay I, Quirke P
Centre for Reproduction, Growth and Development, University of Leeds, Leeds General Infirmary, UK.
Hum Reprod Update. 1996 Mar-Apr;2(2):137-52. doi: 10.1093/humupd/2.2.137.
The analysis of genetic material is fundamental to many medical and scientific applications. One method that has been used extensively for detection or diagnosis of genetic material, especially for single-gene defects or sex, is the polymerase chain reaction (PCR). It is particularly useful when the amount of genetic material is very limited, or samples have been degraded or fixed. In recent years, sensitivity has increased so that even single copies of genes within a single cell can be detected. This article discusses some of the difficulties involved with single-cell PCR and introduces single-cell fluorescent PCR and its potential applications. The use of fluorescent PCR to reduce misdiagnosis is discussed in detail.
遗传物质分析是许多医学和科学应用的基础。一种被广泛用于检测或诊断遗传物质，尤其是单基因缺陷或性别的方法是聚合酶链反应（PCR）。当遗传物质的量非常有限，或者样本已经降解或固定时，它特别有用。近年来，灵敏度有所提高，以至于单个细胞内的单拷贝基因都能被检测到。本文讨论了单细胞PCR所涉及的一些困难，并介绍了单细胞荧光PCR及其潜在应用。详细讨论了使用荧光PCR减少误诊的问题。
