评估四种扩增不同基因组区域的PCR系统用于丙型肝炎病毒感染的分子诊断。（注：原文中是GB virus C，一般译为丙型肝炎病毒，可能存在错误，推测应该是hepatitis C virus，丙肝病毒，按照正确内容翻译了，具体以准确医学知识为准）

Evaluation of four PCR systems amplifying different genomic regions for molecular diagnosis of GB virus C infections.

作者信息

Cantaloube J F, Charrel R N, Attoui H, Biagini P, De Micco P, De Lamballarie X

机构信息

Laboratoire de Biologie Moléculaire, Centre régional de Transfusion Sanguine de Marseille, France.

出版信息

J Virol Methods. 1997 Mar;64(2):131-5. doi: 10.1016/s0166-0934(96)02154-4.

DOI:10.1016/s0166-0934(96)02154-4

PMID:9079759

Abstract

Diagnosis of GB virus C (GBV-C) infection is based on RT-PCR methodology that detects genomic viral RNA. Four sets of primers located in different genomic regions were compared. These primers were used to amplify a reference panel of sera used by the French blood banks, including five positive sera, five negative sera and 10-fold serial dilutions of one positive serum. Three sets of primers located in the 5'UTR, NS3 and NS5a genomic regions gave comparable results with undiluted sera. When diluted sera were tested, the most sensitive protocol was that using a set of primers and a probe selected within the 5'UTR, together with a colorimetric detection based on DNA enzyme immunoassay.

摘要

丙型肝炎病毒（GBV-C）感染的诊断基于检测基因组病毒RNA的逆转录聚合酶链反应（RT-PCR）方法。对位于不同基因组区域的四组引物进行了比较。这些引物用于扩增法国血库使用的一组参考血清，包括五份阳性血清、五份阴性血清以及一份阳性血清的10倍系列稀释液。位于5'非翻译区（5'UTR）、非结构蛋白3（NS3）和非结构蛋白5a（NS5a）基因组区域的三组引物对未稀释血清产生了可比的结果。当检测稀释血清时，最敏感的方案是使用一组位于5'UTR内的引物和探针，以及基于DNA酶免疫测定的比色检测。