Ghiringhelli P D, Albarino C G, Piboul M, Romanowski V
Instituto de Bioquimica y Biologia Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.
Am J Trop Med Hyg. 1997 Feb;56(2):216-25. doi: 10.4269/ajtmh.1997.56.216.
A live attenuated virus vaccine has been recently developed to prevent Argentine hemorrhagic fever. In this paper, we report the nucleotide sequence of the glycoprotein precursor gene (GPC) of the Junin virus vaccine strain (Candid #1) and its flanking untranslated regions. The untranslated regions flanking the GPC genes of different arenaviruses are variable in length, sequence, and secondary structure. However, when this highly attenuated Junin virus strain is compared with the MC2 strain, which is of intermediate virulence, one nucleotide insertion and four nucleotide substitutions are found at positions that do not affect the predicted secondary structure. When Candid #1 and MC2 RNAs are compared, the nucleotide sequence changes in the GPC open reading frame are concentrated in the amino-proximal and the carboxy-proximal regions. The comparison of the amino acid residues shows that the major changes are located in the amino-proximal region of the GPC.
最近开发了一种减毒活病毒疫苗来预防阿根廷出血热。在本文中，我们报告了胡宁病毒疫苗株（Candid #1）糖蛋白前体基因（GPC）及其侧翼非翻译区的核苷酸序列。不同沙粒病毒GPC基因侧翼的非翻译区在长度、序列和二级结构上存在差异。然而，当将这种高度减毒的胡宁病毒株与中等毒力的MC2株进行比较时，发现在不影响预测二级结构的位置有一个核苷酸插入和四个核苷酸替换。当比较Candid #1和MC2的RNA时，GPC开放阅读框中的核苷酸序列变化集中在氨基近端和羧基近端区域。氨基酸残基的比较表明，主要变化位于GPC的氨基近端区域。
