Frölichsthal P, Piatti E
Laboratorio di Neurofarmacologia Sprimentale, Istituto Nazionale Neurologico C. Besta, Milano.
Boll Chim Farm. 1996 Oct;135(9):541-5.
We have evaluated the genotoxic effect of the three organophosphorous (OPs) insecticides dimethoate (DIM), azinphosmethyl (AZN) and diazinon (DIA) or in combination, at a ratio frequently found in foodstuffs by residual analysis. We have carried out the micronucleus (MN) test on cultured rat hepatocytes which were induced to proliferate by epidermal growth factor (EGF), and treated with the insecticides for 48 hours. The doses tested were chosen on the basis of cytotoxicity tests such as Trypan Blue exclusion and mitotic index inhibition. The results showed that AZN was the most toxic and DIM the least toxic compound. None of the compounds tested showed a genotoxic effect when examined separately. Conversely, a significant increase in MN frequency was observed when the three OPs were used in combination at the lowest concentration used when each compound was tested alone.
我们通过残留分析评估了三种有机磷（OPs）杀虫剂乐果（DIM）、甲基毒死蜱（AZN）和二嗪农（DIA）单独或组合使用时（以食品中常见比例）的遗传毒性作用。我们对经表皮生长因子（EGF）诱导增殖的培养大鼠肝细胞进行了微核（MN）试验，并将肝细胞用杀虫剂处理48小时。测试剂量是根据细胞毒性试验（如台盼蓝排斥试验和有丝分裂指数抑制试验）来选择的。结果表明，AZN是毒性最强的化合物，而DIM是毒性最弱的化合物。单独检测时，所测试的化合物均未显示出遗传毒性作用。相反，当三种OPs以各自单独测试时所用的最低浓度组合使用时，观察到微核频率显著增加。
