Kempisty C C, Zak B
Clin Chim Acta. 1977 Oct 1;80(1):187-92. doi: 10.1016/0009-8981(77)90278-9.
A method for the direct determination of methemalbumin in serum by means of difference scanning spectrophotometry is described. The advantages of this quantification technique resides in the ability to obviate unequal background characteristics such as turbidity in one treated sample portion, the avoidance of subtractive measurement on the sides of steep spectra, and a qualitative observation simultaneously derived from the single difference scan. A measure of the stability of the prepared standards is described along with a minimized use of sample and reagent volumes if the latter is desired.
描述了一种通过差示扫描分光光度法直接测定血清中高铁血红蛋白的方法。这种定量技术的优点在于能够消除不相等的背景特征，如一个处理过的样品部分中的浊度，避免在陡峭光谱两侧进行减法测量，以及从单次差示扫描中同时获得定性观察结果。如果需要，还描述了所制备标准品的稳定性测量方法以及最小化样品和试剂体积的使用。
