Wiegand P, Domhöver J, Brinkmann B
Institut für Rechtsmedizin, Westfälische Wilhelms-Universität, Münster.
Pathologe. 1996 Nov;17(6):451-4. doi: 10.1007/s002920050185.
The intensity of DNA degradation in fixed tissues is dependent on the fixation solution and the fixation time. The aim of this study was the investigation of DNA degradation over fixation times of up to 70 days in different tissues (muscle, brain, liver, bone) and with different formalin concentration (2%, 4%, 8%; unbuffered). An additional test was performed to see whether the fixed tissues could be individualized using PCR analysis. The smallest amounts of DNA were extracted from liver and brain and the largest from muscle and bone. The amount of DNA that could be extracted decreased with increasing formalin concentration, while at the same time DNA degradation increased. With the PCR-VNTR system HUMTH01, all fixed samples could be typed regardless of the fixation time and the formalin concentration.
固定组织中DNA降解的强度取决于固定液和固定时间。本研究的目的是调查不同组织（肌肉、脑、肝脏、骨骼）在长达70天的固定时间内以及使用不同福尔马林浓度（2%、4%、8%；未缓冲）时的DNA降解情况。还进行了一项额外测试，以观察能否通过PCR分析对固定组织进行个体化识别。从肝脏和脑中提取的DNA量最少，从肌肉和骨骼中提取的量最多。随着福尔马林浓度的增加，可提取的DNA量减少，同时DNA降解增加。使用PCR-VNTR系统HUMTH01，所有固定样本均可分型，无论固定时间和福尔马林浓度如何。
