Liu W J, Gottshall S L, Hansen P J
Department of Dairy and Poultry Sciences, University of Florida, Gainesville 32611-0902, USA.
Am J Reprod Immunol. 1997 Feb;37(2):199-205. doi: 10.1111/j.1600-0897.1997.tb00214.x.
In sheep, gamma delta T-cell receptor (TCR)+ cells are a major lymphocyte subpopulation in the luminal epithelium of the uterine endometrium. During late pregnancy, this population of T cells increases in number and becomes more granulated. This study was performed to determine whether this apparent activation was induced by local effects of the conceptus or systemic effects of pregnancy.
The unilaterally-pregnant ewe, in which the conceptus is surgically confined to one uterine horn, was used to distinguish between systemic and local effects of pregnancy on function of endometrial gamma delta TCR+ intraepithelial lymphocytes. Lymphocytes collected from peripheral blood, and from the endometrial luminal epithelium of cyclic and unilaterally-pregnant ewes (day 140 of gestation) were analyzed by flow cytometry.
As compared to gamma delta TCR+ lymphocytes in peripheral blood, gamma delta TCR+ intraepithelial lymphocytes from non-pregnant ewes had lower percentage of cells staining positive for CD25, CD44, and L-selectin. There was no effect of pregnancy on the percentage of gamma delta TCR+ peripheral blood lymphocytes staining positive for CD25, CD44, CD29, or L-selectin. Similarly, the percentage of intraepithelial lymphocytes staining positive for these antigens was similar for cells collected from cyclic ewes and cells from the nonpregnant uterine horn of unilaterally-pregnant ewes. In contrast, there was a higher percentage of CD25, CD44, CD29, and L-selectin+ cells for gamma delta TCR+ intraepithelial lymphocytes from the conceptus-containing uterine horn of pregnant ewes than for gamma delta TCR+ intraepithelial lymphocytes from the endometrium of cyclic ewes or from the nonpregnant uterine horn of pregnant ewes.
The local presence of the conceptus causes changes in cell surface marker expression on endometrial gamma delta TCR+ intraepithelial lymphocytes during pregnancy. These changes may reflect conceptus-induced activation of this population of cells.
在绵羊中，γδT细胞受体（TCR）+细胞是子宫内膜腔上皮中的主要淋巴细胞亚群。在妊娠后期，这群T细胞数量增加且颗粒增多。本研究旨在确定这种明显的激活是由孕体的局部效应还是妊娠的全身效应所诱导。
使用单侧妊娠母羊，通过手术将孕体限制在一个子宫角内，以区分妊娠对子宫内膜γδTCR +上皮内淋巴细胞功能的全身和局部效应。通过流式细胞术分析从外周血以及周期性和单侧妊娠母羊（妊娠第140天）的子宫内膜腔上皮中收集的淋巴细胞。
与外周血中的γδTCR +淋巴细胞相比，未妊娠母羊的γδTCR +上皮内淋巴细胞中CD25、CD44和L-选择素染色阳性的细胞百分比更低。妊娠对γδTCR +外周血淋巴细胞中CD25、CD44、CD29或L-选择素染色阳性的百分比没有影响。同样，从周期性母羊收集的细胞以及单侧妊娠母羊未妊娠子宫角的细胞中，这些抗原染色阳性的上皮内淋巴细胞百分比相似。相比之下，妊娠母羊含孕体子宫角的γδTCR +上皮内淋巴细胞中CD25、CD44、CD29和L-选择素+细胞的百分比高于周期性母羊子宫内膜或妊娠母羊未妊娠子宫角的γδTCR +上皮内淋巴细胞。
孕体的局部存在导致妊娠期间子宫内膜γδTCR +上皮内淋巴细胞的细胞表面标志物表达发生变化。这些变化可能反映了孕体诱导的这群细胞的激活。
