Inaba T, Saito H, Fukushima R, Hashiguchi Y, Lin M T, Inoue T, Fukatsu K, Muto T, Oka T, Takenaka A, Takahashi S, Noguchi T
Department of Surgery I, Faculty of Medicine, University of Tokyo, Japan.
JPEN J Parenter Enteral Nutr. 1997 Mar-Apr;21(2):55-62. doi: 10.1177/014860719702100255.
Both growth hormone (GH) and insulin-like growth factor 1 (IGF-1) improve protein metabolism after surgical insult in subjects without liver disease. However, these effects in chronic liver injury, in which the GH-IGF-1 axis is impaired, have not been investigated. We examined the anabolic effects of GH and IGF-1 after gastrectomy in rats with chronic mild liver injury.
Rats with chronic mild liver injury induced by thioacetamide were used. After gastrectomy, the rats were randomized into vehicle control, GH, and IGF-1 groups. In the latter two groups, 0.8 IU/kg/d of GH or 4 mg/kg/d of IGF-1 was infused for 72 hours. Anabolic effects were assessed by body weight change, 3-methylhistidine (3-MH) excretion, nitrogen excretion, and whole-body protein turnover. Organ weights, plasma levels of glucose, insulin, and IGF-1, tissue IGF-1 levels, hepatic messenger RNA (mRNA) content, and intestinal structure were also determined.
Both GH and IGF-1 decreased nitrogen excretion. IGF-1, but not GH, increased postoperative body weight, whole-body protein turnover, and splenic weight. IGF-1 reduced atrophy of the intestinal mucosa. GH treatment increased hepatic IGF-1-mRNA and the plasma IGF-1 level, whereas IGF-1 treatment increased the plasma IGF-1 level with no change in the hepatic IGF-1-mRNA content. There were no significant differences in plasma glucose or insulin levels among the three groups. Neither GH nor IGF-1 affected the gastrocnemius muscle IGF-1 level.
IGF-1 has beneficial effects, whereas GH has only limited effects on post-operative protein metabolism, gut integrity, and splenic weight in chronic mild liver injury.
生长激素（GH）和胰岛素样生长因子1（IGF-1）均可改善无肝脏疾病受试者在手术创伤后的蛋白质代谢。然而，在生长激素-胰岛素样生长因子1（GH-IGF-1）轴受损的慢性肝损伤中，这些作用尚未得到研究。我们研究了慢性轻度肝损伤大鼠胃切除术后生长激素（GH）和胰岛素样生长因子1（IGF-1）的合成代谢作用。
采用硫代乙酰胺诱导的慢性轻度肝损伤大鼠。胃切除术后，将大鼠随机分为溶剂对照组、生长激素组和胰岛素样生长因子1组。在后两组中，以0.8 IU/kg/d的生长激素或4 mg/kg/d的胰岛素样生长因子1输注72小时。通过体重变化、3-甲基组氨酸（3-MH）排泄、氮排泄和全身蛋白质周转率评估合成代谢作用。还测定了器官重量、血浆葡萄糖、胰岛素和胰岛素样生长因子1水平、组织胰岛素样生长因子1水平、肝脏信使核糖核酸（mRNA）含量和肠道结构。
生长激素（GH）和胰岛素样生长因子1（IGF-1）均降低了氮排泄。胰岛素样生长因子1（IGF-1）而非生长激素（GH）增加了术后体重、全身蛋白质周转率和脾脏重量。胰岛素样生长因子1（IGF-1）减轻了肠黏膜萎缩。生长激素（GH）治疗增加了肝脏胰岛素样生长因子1-mRNA和血浆胰岛素样生长因子1水平，而胰岛素样生长因子1（IGF-1）治疗增加了血浆胰岛素样生长因子1水平，肝脏胰岛素样生长因子1-mRNA含量无变化。三组间血浆葡萄糖或胰岛素水平无显著差异。生长激素（GH）和胰岛素样生长因子1（IGF-1）均未影响腓肠肌胰岛素样生长因子1水平。
在慢性轻度肝损伤中，胰岛素样生长因子1（IGF-1）具有有益作用，而生长激素（GH）对术后蛋白质代谢、肠道完整性和脾脏重量的影响有限。
