Rupnik M, Braun V, Soehn F, Janc M, Hofstetter M, Laufenberg-Feldmann R, von Eichel-Streiber C
Verfugungsgebäude für Forschung und Entwicklung, Johannes Gutenberg-Universität Mainz, Germany.
FEMS Microbiol Lett. 1997 Mar 15;148(2):197-202. doi: 10.1111/j.1574-6968.1997.tb10288.x.
We have used six independent polymerase chain reactions (A1-A3 and B1-B3) for amplification of the entire sequence of the two toxin genes tcdA and tcdB of several Clostridium difficile strains. With this approach we have detected (1) restriction site polymorphisms which are distributed all over the genes, and (2) deletions that could be found only in tcdA. Characteristic differences between strains were mainly focused to the 5' third of tcdB (B1 fragment) and/or the 3' third of tcdA (A3 fragment). The possible use of our approach for typing of C. difficile toxin genes is discussed.
我们使用了六个独立的聚合酶链反应（A1 - A3和B1 - B3）来扩增几种艰难梭菌菌株的两个毒素基因tcdA和tcdB的全序列。通过这种方法，我们检测到：（1）遍布基因的限制性位点多态性，以及（2）仅在tcdA中发现的缺失。菌株之间的特征性差异主要集中在tcdB的5'端三分之一（B1片段）和/或tcdA的3'端三分之一（A3片段）。本文讨论了我们的方法在艰难梭菌毒素基因分型中的可能应用。
