Sébédio J L, Juanéda P, Dobson G, Ramilison I, Martin J C, Chardigny J M, Christie W W
INRA, Unité de Nutrition Lipidique, Dijon, France.
Biochim Biophys Acta. 1997 Mar 10;1345(1):5-10. doi: 10.1016/s0005-2760(97)00015-5.
Metabolism of conjugated isomers of linoleic acid (CLA) in rats was studied by feeding high quantities of CLA (180 mg/day) for six days to animals that had been reared on a fat-free diet for two weeks. After this period, animals were sacrificed and liver lipids extracted. High-performance liquid chromatography (HPLC) analyses with UV detection revealed the presence of conjugated polyunsaturated fatty acids in the total liver lipid methyl esters. After isolation by HPLC, three fatty acid metabolites were identified by gas liquid chromatography coupled with mass spectrometry as being C20:3 delta 8,12,14, C20:4 delta 5,8,12,14 and C20:4 delta 5,8,11,13. A higher quantity of C20:4 delta 5,8,12,14 compared to C20:4 delta 5,8,11,13 was observed. These must arise from the elongation and desaturation of 18:2 delta 10,12 and 18:2 delta 9,11, respectively.
通过给在无脂饮食中饲养两周的大鼠连续六天喂食大量共轭亚油酸（CLA，180毫克/天），研究了大鼠体内CLA共轭异构体的代谢情况。在此期间过后，处死动物并提取肝脏脂质。采用紫外检测的高效液相色谱（HPLC）分析显示，在总肝脏脂质甲酯中存在共轭多不饱和脂肪酸。通过HPLC分离后，利用气相色谱-质谱联用鉴定出三种脂肪酸代谢产物，分别为C20:3δ8,12,14、C20:4δ5,8,12,14和C20:4δ5,8,11,13。观察到C20:4δ5,8,12,14的含量高于C20:4δ5,8,11,13。这些代谢产物必定分别源自18:2δ10,12和18:2δ9,11的延长和去饱和作用。
