大鼠体内α-亚麻酸共轭异构体（CLnA）的代谢产物
Metabolites of conjugated isomers of alpha-linolenic acid (CLnA) in the rat.
作者信息
Destaillats Frédéric, Berdeaux Olivier, Sébédio Jean-Louis, Juaneda Pierre, Grégoire Stéphane, Chardigny Jean-Michel, Bretillon Lionel, Angers Paul
机构信息
Department of Food Science and Nutrition and Dairy Research Center (STELA), Université Laval, Sainte Foy, Québec, Canada.
出版信息
J Agric Food Chem. 2005 Mar 9;53(5):1422-7. doi: 10.1021/jf0481958.
Rumelenic (cis-9,trans-11,cis-15 18:3) acid is a naturally occurring conjugated isomer of alpha-linolenic acid (CLnA) in milk fat. Metabolism in rats was studied using a synthetic CLnA mixture, composed mainly by equimolar quantities of cis-9,trans-11,cis-15 and cis-9,trans-13,cis-15 CLnA isomers. Their metabolisms were studied by feeding high quantities of CLnA (150 mg/day) for 4 days to rats that had been reared on a fatfree diet for 2 weeks. After this period, animals were sacrificed and liver and epididymal adipose tissue lipids extracted. Six metabolites of the cis-9,trans-11,cis-15 18:3 CLnA isomers were identified as being cis-7,trans-9,cis-13 16:3, cis-11,trans-13,cis-17 20:3, cis-8,cis-11,trans-13,cis-17 20:4, cis-5,cis-8,cis-11,trans-13,cis-17 20:5, cis-7,cis-10,cis-13,trans-15,cis-19 22:5, and cis-4,cis-7,cis-10,cis-13,trans-15,cis-19 22:6 acids. Two metabolites of cis-9,trans-13,cis-15 18:3 CLnA isomer were also identified by GC-MS as being cis-7,trans-11,cis-13 16:3 and cis-5,cis-8,cis-11,trans-15,cis-17 20:5.
鲁梅勒尼克酸（顺-9，反-11，顺-15 18:3）是乳脂肪中天然存在的α-亚麻酸（CLnA）的共轭异构体。使用主要由等摩尔量的顺-9，反-11，顺-15和顺-9，反-13，顺-15 CLnA异构体组成的合成CLnA混合物研究大鼠体内的代谢情况。通过向在无脂饮食中饲养2周的大鼠喂食大量CLnA（150毫克/天），持续4天，来研究它们的代谢情况。在此期间过后，处死动物并提取肝脏和附睾脂肪组织脂质。顺-9，反-11，顺-15 18:3 CLnA异构体的六种代谢产物被鉴定为顺-7，反-9，顺-13 16:3、顺-11，反-13，顺-17 20:3、顺-8，顺-11，反-13，顺-17 20:4、顺-5，顺-8，顺-11，反-13，顺-17 20:5、顺-7，顺-10，顺-13，反-15，顺-19 22:5和顺-4，顺-7，顺-10，顺-13，反-15，顺-19 22:6酸。顺-9，反-13，顺-15 18:3 CLnA异构体的两种代谢产物也通过气相色谱-质谱联用仪鉴定为顺-7，反-11，顺-13 16:3和顺-5，顺-8，顺-11，反-15，顺-17 20:5。
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