Nathanson S D, Anaya P, Karvelis K C, Eck L, Havstad S
Department of Surgery, Case Western Reserve University at Henry Ford Health System, Detroit, Michigan, USA.
Ann Surg Oncol. 1997 Mar;4(2):104-10. doi: 10.1007/BF02303791.
The study was designed to compare the uptake of technetium-99m-labeled human serum albumin ([99mTc]HSA) and 99mtechnetium-labeled sulfur colloid ([99mTc]SC) in the sentinel lymph nodes (SLNs).
Radiocolloid levels in the SLN, femoral lymph node (FLN), ischial lymph node (ILN), spleen (SP), and right rear footpad (RRF) were quantitated over a period of 240 min after injection of [99mTc]SC or [99mTc]HSA into the rear footpad of C57 BL/6 mice.
There was a significantly greater (p < 0.001) radioactive count in SLNs after [99mTc]SC (mean cpm 211,084.8) compared to [99mTc]HSA (mean cpm 115,640.8). In contrast, the counts in the FLNs were higher after [99mTc]HSA (mean cpm 11,333.4) than after [99mTc]SC injection (mean cpm 5,065.5). The percent uptake in the SP was higher after [99mTc]HSA than after [99mTc]SC injection.
[99mTc]SC is rapidly and efficiently taken up by lymphatics at the primary injection site, is significantly retained in the SLN, and flows slowly to the next echelon node (FLN or ILN) and to the systemic circulation (SP). [99mTc]HSA tends to accumulate less efficiently in the SLN and to disperse more rapidly to the next echelon nodes and to the systemic circulation. By extrapolation, [99mTc]SC is likely to be a better radiocolloid for the intraoperative detection of SLNs.
本研究旨在比较锝-99m标记的人血清白蛋白（[99mTc]HSA）和锝-99m标记的硫胶体（[99mTc]SC）在前哨淋巴结（SLN）中的摄取情况。
将[99mTc]SC或[99mTc]HSA注入C57 BL/6小鼠的后足垫，在240分钟内对SLN、股淋巴结（FLN）、坐骨淋巴结（ILN）、脾脏（SP）和右后足垫（RRF）中的放射性胶体水平进行定量分析。
与[99mTc]HSA（平均计数每分钟211,084.8次）相比，[99mTc]SC注射后SLN中的放射性计数显著更高（p < 0.001）。相比之下，[99mTc]HSA注射后FLN中的计数（平均计数每分钟11,333.4次）高于[99mTc]SC注射后（平均计数每分钟5,065.5次）。[99mTc]HSA注射后SP中的摄取百分比高于[99mTc]SC注射后。
[99mTc]SC在初次注射部位被淋巴管快速有效地摄取，在SLN中显著滞留，并缓慢流向次级淋巴结（FLN或ILN）和体循环（SP）。[99mTc]HSA在SLN中的积聚效率较低，且更快地扩散到次级淋巴结和体循环。由此推断，[99mTc]SC可能是术中检测SLN更好的放射性胶体。
