Fiorani M, Biagiarelli B, Vetrano F, Guidi G, Dachà M, Stocchi V
Centro di Biochimica delle Proteine, Università di Urbino, Italy.
Bioelectromagnetics. 1997;18(2):125-31.
The aim of this study was to investigate the effects of 50 Hz magnetic fields (0.2-0.5 mT) on rabbit red blood cells (RBCs) that were exposed simultaneously to the action of an oxygen radical-generating system, Fe(II)/ascorbate. Previous data obtained in our laboratory showed at the exposure of rabbit erythrocytes or reticulocytes to Fe(II)/ascorbate hexokinase inactivation, whereas the other glycolytic enzymes do not show any decay. We also observed depletion of reduced glutathione (GSH) content with a concomitant intracellular and extracellular increase in oxidized glutathione (GSSG) and a decrease in energy charge. In this work we investigated whether 50 Hz magnetic fields could influence the intracellular impairments that occur when erythrocytes or reticulocytes are exposed to this oxidant system, namely, inactivation of hexokinase activity, GSH depletion, a change in energy charge, and hemoglobin oxidation. The results obtained indicate the a 0.5 mT magnetic field had no effect on intact RBCs, whereas it increased the damage with Fe(II)/ascorbate to a 0.5 mT magnetic field induced a significant further decay in hexokinase activity (about 20%) as well as a twofold increase in methemoglobin production compared with RBCs that were exposed to the oxidant system alone. Although further studies will be needed to determine the physiological implications of these data, the results reported in this study demonstrate that the effects of the magnetic fields investigated are able to potentiate the cellular damage induced in vitro by oxidizing agents.
本研究的目的是调查50Hz磁场（0.2 - 0.5mT）对同时暴露于产生活性氧系统（Fe(II)/抗坏血酸盐）作用下的兔红细胞（RBCs）的影响。我们实验室之前获得的数据表明，兔红细胞或网织红细胞暴露于Fe(II)/抗坏血酸盐时己糖激酶失活，而其他糖酵解酶未显示任何衰减。我们还观察到还原型谷胱甘肽（GSH）含量减少，同时氧化型谷胱甘肽（GSSG）在细胞内和细胞外均增加，并且能荷降低。在这项工作中，我们研究了50Hz磁场是否会影响红细胞或网织红细胞暴露于该氧化系统时发生的细胞内损伤，即己糖激酶活性失活、GSH消耗、能荷变化和血红蛋白氧化。获得的结果表明，0.5mT的磁场对完整的RBCs没有影响，而它增加了Fe(II)/抗坏血酸盐对细胞的损伤。与仅暴露于氧化系统的RBCs相比，0.5mT的磁场诱导己糖激酶活性显著进一步衰减（约20%），同时高铁血红蛋白生成增加两倍。尽管需要进一步研究来确定这些数据的生理学意义，但本研究报告的结果表明，所研究的磁场效应能够增强体外氧化剂诱导的细胞损伤。
