Woo J H, Kim Y S, Hwang S D
Department of Internal Medicine, Soon Chun Hyang University, Asan, Korea.
Mol Cells. 1997 Feb 28;7(1):28-33.
Staphylococcus aureus produces various proteins in response to discrete signals from the external environment like many other pathogenic microorganisms. Certain staphylococcal exoproteins including toxic shock syndrome toxin-1 (TSST-1) are secreted according to the stimuli from the environment, and the quantity synthesized is influenced by a number of different parameters. Using a transposon Tn551-mediated mutagenesis, a mutant (RN 6390) defective in TSST-1 from synthesis was constructed. TSST-1 from wild strain and mutant stain were purified and quantitated from culture supernatants of Staphylococcus aureus. The mutant strain RN 6390 produced only 2% of TSST-1 compared with that produced by the wild strain RN4282. Southern blot hybridization with a tst (TSST-1 gene) probe indicated that the inactivated chromosomal locus is distinct from the tst. These results suggest that transposition by Tn551 inactivated a chromosomal locus whose activity was essential for the expression of the TSST-1 gene.
与许多其他致病微生物一样，金黄色葡萄球菌会根据来自外部环境的离散信号产生各种蛋白质。某些葡萄球菌外毒素蛋白，包括毒性休克综合征毒素-1（TSST-1），会根据环境刺激而分泌，其合成量受许多不同参数的影响。利用转座子Tn551介导的诱变，构建了一株在TSST-1合成方面存在缺陷的突变株（RN 6390）。从金黄色葡萄球菌的培养上清液中纯化并定量野生菌株和突变菌株的TSST-1。与野生菌株RN4282相比，突变菌株RN 6390产生的TSST-1仅为其2%。用tst（TSST-1基因）探针进行的Southern印迹杂交表明，失活的染色体位点与tst不同。这些结果表明，Tn551的转座使一个染色体位点失活，该位点的活性对于TSST-1基因的表达至关重要。
