Rho J, Lee S, de Villiers E M, Choe J
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon, South Korea.
Virus Res. 1997 Feb;47(2):155-66. doi: 10.1016/s0168-1702(96)01410-4.
Human papillomavirus type 59 (HPV-59) was cloned from a vulvar intraepithelial neoplasia and the complete nucleotide sequence was determined. This virus is closely related to HPV-18 and -39 (60% homology in nucleotide sequence) and is grouped with the genital HPV types. In the present paper, we demonstrate that the HPV-59 E2 transactivator represses its E6 promoter-mediated transcription. We have also analyzed cis-regulatory elements in the upstream regulatory region (URR) of HPV-59 using chloramphenicol acetyl transferase assays as well as electrophoresis mobility shift assays (EMSA). The results allow for a subdivision of the HPV-59 URR into three regions of activity: distal (nt 7149-7493), central (nt 7493-7742), and proximal (nt 7742-7748). In particular, the 250 bp (nt 7493-7742) of the central region plays an important role as a constitutive enhancer element for the maximal transcription of the E6 promoter. Our results suggest that the transcription factors AP1, Oct1, SP1 and unidentified factors bind to the HPV-59 E6 promoter region, whereas NF1, GRE and TFIID fail to bind despite the presence of putative binding sites in the DNA sequence.
人乳头瘤病毒59型（HPV - 59）是从一例外阴上皮内瘤变中克隆出来的，并测定了其完整的核苷酸序列。该病毒与HPV - 18和 - 39密切相关（核苷酸序列同源性为60%），并被归类为生殖器HPV类型。在本文中，我们证明HPV - 59 E2反式激活因子可抑制其E6启动子介导的转录。我们还使用氯霉素乙酰转移酶分析以及电泳迁移率变动分析（EMSA）对外阴人乳头瘤病毒59型（HPV - 59）上游调控区（URR）中的顺式调控元件进行了分析。结果可将HPV - 59 URR分为三个活性区域：远端（核苷酸7149 - 7493）、中部（核苷酸7493 - 7742）和近端（核苷酸7742 - 7748）。特别是，中部区域的250 bp（核苷酸7493 - 7742）作为E6启动子最大转录的组成型增强子元件发挥着重要作用。我们的结果表明，转录因子AP1、Oct1、SP1和未知因子与HPV - 59 E6启动子区域结合，而尽管DNA序列中存在假定的结合位点，但NF1、GRE和TFIID未能结合。
