Nakagawa M, Stites D P, Farhat S, Sisler J R, Moss B, Kong F, Moscicki A B, Palefsky J M
Department of Laboratory Medicine, School of Medicine, University of California at San Francisco, 94143-0134, USA.
J Infect Dis. 1997 Apr;175(4):927-31. doi: 10.1086/513992.
Cytotoxic T lymphocyte (CTL) responses to the human papillomavirus (HPV) type 16 E6 and E7 proteins were measured in 20 women with known HPV and cervical disease status. CTL assays were performed after stimulation with E6 or E7 fusion proteins using autologous B lymphoblastoid cells infected with vaccinia viruses expressing E6 or E7. CTL responses to E6 and E7 were detected in 6 (75%) of 8 and 5 (56%) of 9 HPV-16-positive women without cervical intraepithelial neoplasia (CIN), respectively. Responses to E6 or E7 were each detected in only 2 (29%) of 7 HPV-16-positive women with CIN. Responses to both antigens were found in 63% of women without CIN and 14% of those with CIN. CTL responses to E6 or E7 are more commonly detectable in HPV-16-positive women without CIN than in HPV-16-positive women with CIN, suggesting that CTL response may play a role in disease protection.
在20名已知人乳头瘤病毒（HPV）感染情况及宫颈疾病状态的女性中，检测了细胞毒性T淋巴细胞（CTL）对16型人乳头瘤病毒（HPV）E6和E7蛋白的反应。使用表达E6或E7的痘苗病毒感染自体B淋巴母细胞，在用E6或E7融合蛋白刺激后进行CTL检测。在8名无宫颈上皮内瘤变（CIN）的HPV - 16阳性女性中，分别有6名（75%）和9名中的5名（56%）检测到对E6和E7的CTL反应。在7名患有CIN的HPV - 16阳性女性中，仅各有2名（29%）检测到对E6或E7的反应。在无CIN的女性中，63%的人检测到对两种抗原的反应，而在患有CIN的女性中这一比例为14%。与患有CIN的HPV - 16阳性女性相比，在无CIN的HPV - 16阳性女性中更常检测到对E6或E7的CTL反应，这表明CTL反应可能在疾病保护中发挥作用。
