Klyce S D
Invest Ophthalmol Vis Sci. 1977 Oct;16(10):968-73.
Swollen rabbit corneas incubated in vitro with their posterior surfaces blocked with silicone oil maintained fairly constant thickness over an 11 hr. period. Increasing the simulated intraocular pressure from 10 to 30 mm. Hg did not produce stromal thinning. When theophylline was added to stimulate epithelial Cl secretion by increasing the Cl permeability of the tear-facing epithelial membrane, corneas thinned at the average of 1.3 micrometer/hr. over a 6 1/2 hr. period. When the epithelial perfusion solution was made Cl-free by SO4 substitution to favor the passive flow of Cl from the cells to the tear solution, thinning of 3.91 micrometer/hr. over a 7 hr. period was observed. When corneas were perfused with Cl-free medium plus theophylline, thinning at the average rate of 6.20 micrometer/hr. over an 8 hr. period was achieved. Therefore the corneal epithelium is capable of thinning a swollen stroma by transport of fluid coupled to its Cl secretion, which can be enhanced by simple substitutions in the tear-side bathing solution.
在体外培养的肿胀兔角膜，其后表面用硅油封闭，在11小时内厚度保持相当恒定。将模拟眼内压从10毫米汞柱提高到30毫米汞柱并未导致基质变薄。当加入茶碱以通过增加面向泪液的上皮细胞膜的氯离子通透性来刺激上皮细胞氯离子分泌时，角膜在6个半小时内平均每小时变薄1.3微米。当通过用硫酸根替代使上皮灌注液无氯离子，以利于氯离子从细胞被动流向泪液时，观察到在7小时内每小时变薄3.91微米。当用无氯离子培养基加茶碱灌注角膜时，在8小时内平均每小时变薄6.20微米。因此，角膜上皮能够通过与其氯离子分泌相关的液体转运使肿胀的基质变薄，通过在泪液侧冲洗液中进行简单替代可增强这种转运。
