Saxon B R, Byard R W, Han P
Department of Haematology, Women's and Children's Hospital, North Adelaide, Australia.
Pediatr Pathol Lab Med. 1997 Mar-Apr;17(2):259-66.
Tumor invasion and metastasis are believed to involve the adhesive interaction of tumor cells with extracellular matrix (ECM). This study reports the expression of laminin, fibronectin, thrombospondin, tenascin, and CD44 in rhabdomyosarcoma cells taken from tumors derived from 12 pediatric patients. Twelve paraffin-embedded rhabdomyosarcomas consisting of five alveolar, six embryonal, and one botryoid subtype were examined. All tumors demonstrated positive staining for tenascin and thrombospondin, 10 were positive for fibronectin, 5 positive for laminin, and 4 positive for CD44. Both specimens without cellular staining for fibronectin were of embryonal subtype and no alveolar rhabdomyosarcomas were positive for CD44. No association of the expression of the studied ECM proteins and receptor with the presence of metastatic disease at clinical presentation or with the level of tumor differentiation was found.
肿瘤侵袭和转移被认为涉及肿瘤细胞与细胞外基质（ECM）的黏附相互作用。本研究报告了从12例儿科患者的肿瘤中获取的横纹肌肉瘤细胞中层粘连蛋白、纤连蛋白、血小板反应蛋白、腱生蛋白和CD44的表达情况。对12个石蜡包埋的横纹肌肉瘤进行了检查，其中包括5个肺泡型、6个胚胎型和1个葡萄簇型亚型。所有肿瘤对腱生蛋白和血小板反应蛋白呈阳性染色，10个对纤连蛋白呈阳性，5个对层粘连蛋白呈阳性，4个对CD44呈阳性。两个无纤连蛋白细胞染色的标本均为胚胎型亚型，且无肺泡型横纹肌肉瘤对CD44呈阳性。未发现所研究的ECM蛋白和受体的表达与临床表现时转移疾病的存在或肿瘤分化程度之间存在关联。
