Kesari R, Rai M, Gupta V K
Pt. Ravishankar Shukla University, School of Studies in Chemistry, Raipur, India.
J AOAC Int. 1997 Mar-Apr;80(2):388-91.
A sensitive spectrophotometric method was developed for determination of paraquat, a widely used herbicide. Paraquat was reduced with glucose in an alkaline medium, and the blue radical ion obtained was measured at 600 nm. Beer's law was obeyed at 0.1-1.2 ppm paraquat. The molar absorptivity was 1.26 x 10(5) L mol-1 cm-1. The standard deviation and relative standard deviation were +/- 0.007 and 2.0%, respectively, for 5 micrograms paraquat/10 mL analyzed over 7 days. The method was free from interference by other commonly used pesticides and metal ions. The method may be used to the determine paraquat in plants, fruits, grains, water, blood, and urine.
开发了一种灵敏的分光光度法用于测定百草枯，一种广泛使用的除草剂。百草枯在碱性介质中用葡萄糖还原，在600 nm处测量得到的蓝色自由基离子。在百草枯浓度为0.1 - 1.2 ppm时符合比尔定律。摩尔吸光系数为1.26×10(5) L mol-1 cm-1。在7天内对5微克百草枯/10毫升进行分析时，标准偏差和相对标准偏差分别为±0.007和2.0%。该方法不受其他常用农药和金属离子的干扰。该方法可用于测定植物、水果、谷物、水、血液和尿液中的百草枯。
