实验室饲养的索诺拉库蠓体内的蓝舌病毒：斑点印迹法、酶联免疫吸附测定法及免疫电子显微镜技术的应用

Bluetongue virus in laboratory-reared Culicoides variipennis sonorensis: applications of dot-blot, ELISA, and immunoelectron microscopy.

作者信息

Nunamaker R A, Mecham J O, Wigington J G, Ellis J A

机构信息

Arthropod-Borne Animal Diseases Research Laboratory, USDA-ARS, Laramie, WY 82071, USA.

出版信息

J Med Entomol. 1997 Jan;34(1):18-23. doi: 10.1093/jmedent/34.1.18.

DOI:10.1093/jmedent/34.1.18

PMID:9086705

Abstract

An avidin-biotin complex (ABC) dot-blot, an antigen capture enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy (IEM) were used to detect bluetongue (BLU) virus or viral antigen or both in adult Culicoides variipennis sonorensis Wirth & Jones. The dot-blot and ELISA procedures detected viral antigen in 10-22% (depending on serotype) of the biting midges infected with BLU-2, BLU-10, BLU-13, and BLU-17 and approximately 68% of the midges infected with BLU-11. IEM analyses revealed BLU virus in salivary glands, fat body, and thoracic muscle tissue from infected insects. There appeared to be selective growth of the virus in salivary gland tissue.

摘要

采用抗生物素蛋白-生物素复合物（ABC）斑点印迹法、抗原捕获酶联免疫吸附测定（ELISA）和免疫电子显微镜（IEM）检测成年索诺拉库蠓（Culicoides variipennis sonorensis Wirth & Jones）体内的蓝舌病（BLU）病毒、病毒抗原或两者。斑点印迹法和ELISA检测程序在感染BLU-2、BLU-10、BLU-13和BLU-17的叮咬蠓中，检测到10%-22%（取决于血清型）的病毒抗原，在感染BLU-11的蠓中约检测到68%的病毒抗原。IEM分析在受感染昆虫的唾液腺、脂肪体和胸肌组织中发现了蓝舌病病毒。病毒似乎在唾液腺组织中选择性生长。