Kobayashi K
Osaka Prefectural Institute of Public Health.
Nihon Rinsho. 1997 Mar;55(3):675-9.
Bacterial agglutination(BA) test was used to detect anti-E. coli somatic(O26, O111, O157) antibodies. A total of 355-serum samples were collected from 45 patients were positive by culture for E. coli O157:H7 (group I), from 47 HUS patients without EHEC(group II), from 50 family members of group I and II, from 22 patients of diarrhea with or without blood and from 23 of healthy controls. Sera from 41(91.1%) of the group I, from 34(72.3%) of the group II, from 8(16.0%) of the group III, from 2(33.3%) patients with bloody diarrhea(6 patients) had significant antibody levels(1:160 < or =) to the O157-antigen. Only one patient implicated HUS and without any EHEC had a titer of positive level to the O111-antigen. These sera with significant levels of antibody were revealed at 5th to 6th day of illness, and continued to 45 or more days of illness. Twenty-five(50%) from whom stool specimens were negative by culture for E. coli O157 in the group III had median antibody levels (1:40 approximately 1:80). This group had high antibody levels than two groups of healthy controls and the patients with other pathogen than E. coli O157. These results suggest that high antibody levels members were infected with E. coli O157, and also indicate that BA test of sera from patients with some enteric signs provided valuable for confirmation and diagnosis of the EHEC infection.
采用细菌凝集试验（BA）检测抗大肠杆菌菌体（O26、O111、O157）抗体。共收集了355份血清样本，其中45份来自经培养确诊为大肠杆菌O157:H7阳性的患者（第一组），47份来自无肠出血性大肠杆菌（EHEC）的溶血尿毒综合征（HUS）患者（第二组），50份来自第一组和第二组患者的家庭成员，22份来自有或无便血的腹泻患者，以及23份来自健康对照者。第一组41例（91.1%）、第二组34例（72.3%）、第三组8例（16.0%）、便血腹泻患者中的2例（6例患者中的33.3%）血清对O157抗原具有显著抗体水平（1:160及以上）。仅1例无任何EHEC但患有HUS的患者对O111抗原呈阳性滴度。这些具有显著抗体水平的血清在发病第5至6天出现，并持续至发病45天或更久。第三组中25例（50%）粪便标本经培养未检出大肠杆菌O157的患者，其抗体水平中位数为（1:40至1:80）。该组的抗体水平高于两组健康对照者以及感染非大肠杆菌O157其他病原体的患者。这些结果表明，抗体水平高的人群感染了大肠杆菌O157，同时也表明对有某些肠道症状患者的血清进行BA试验，对确诊和诊断EHEC感染具有重要价值。
